食品科学

• 分析检测 • 上一篇    下一篇

脂肪酶催化竹叶抗氧化物中异荭草苷和异牡荆苷酰化

马 祥,晏日安,逯与运   

  1. 暨南大学食品科学与工程系,广东 广州 510632
  • 出版日期:2013-08-25 发布日期:2013-09-03

Candida antarctica Lipase B-Catalyzed Acylation of Isoorientin and Isovitexin in Antioxidant of Bamboo Leaves (AOB)

MA Xiang,YAN Ri-an,LU Yu-yun   

  1. Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China
  • Online:2013-08-25 Published:2013-09-03

摘要:

研究南极假丝酵母脂肪酶在叔戊醇中催化竹叶抗氧化物中异荭草苷和异牡荆苷的酰化。反应条件为脂肪酶用量50g/L、分子筛4Å用量100g/L、竹叶抗氧化物中黄酮苷与月桂酸的物质的量比1:4、反应温度60℃、搅拌转速150r/min、反应时间48h。经过高效液相色谱分析,制备液相分离,得到2种被酰化的黄酮苷。对产物的结构采用电喷雾电离质谱分析,1H-NMR及13C-NMR表征,确定产物分别为异荭草苷-6″-月桂酸酯和异牡荆苷-6″-月桂酸酯。

关键词: 脂肪酶, 异荭草苷, 异牡荆苷, 高效液相色谱, 酰化

Abstract:

The acylation catalyzed by Candida antarctica lipase B of isoorientin and isovitexin in antioxidant of bamboo
leaves (AOB) in tert-amyl alcohol as reaction medium was studied. The reaction condition was as follows: 50 g/L
lipase,100 g/L molecular sieve 4 Å, a molar ratio of flavonoids in AOB to laurate acid of 1:4, 60 ℃, 150 r/min stirring and
a reaction duration of 48 h. After being analyzed and separated by high-performance liquid chromatography, two types of
acylated flavonoids were obtained. Confirmation of chemical structures was performed by ESI-MS, 1H-NMR and 13C-NMR
and the two types of acylated flavonoids were isoorientin-6″-laurate and isovitexin-6″-laurate.

Key words: lipase, isoorientin, isovitexin, high performance liquid chromatography, acylation