食品科学

• 营养卫生 • 上一篇    下一篇

白腐真菌产木聚糖酶降解半纤维素及改善肠道微生态平衡作用

李天歌1,岳晓禹2,3,*,李自刚2,蔡青和3   

  1. 1.四川农业大学食品学院,四川 雅安 625014;2.河南牧业经济学院质检系,河南 郑州 450011;
    3.中国农业大学食品科学与营养工程学院,北京 100083
  • 出版日期:2013-09-15 发布日期:2013-09-27
  • 通讯作者: 岳晓禹
  • 基金资助:

    2011年河南省重点科技发展计划项目(112102110034);2011年度河南省教育厅自然科学研究计划项目(2011A550014;12A550007);
    河南牧业经济学院博士科研启动资金项目(5040190)

Xylanase from Pleurotus ostreatus SYJ-042 Degrades Hemicellulose and Ameliorates Intestinal Microeological Balance

LI Tian-ge1,YUE Xiao-yu2,3,*,LI Zi-gang2,CAI Qing-he3   

  1. 1. College of Food Science, Sichuan Agricultural University, Ya’an 625014, China;
    2. Department of Quality Detection and Management, Henan College of Animal Husbandry and Economy, Zhengzhou 450011, China;
    3. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
  • Online:2013-09-15 Published:2013-09-27
  • Contact: YUE Xiao-yu

摘要:

以白腐真菌菌株Pleurotus ostreatus SYJ-042为研究对象,对该菌株产木聚糖酶进行果皮和动物饲用效果研究。果皮效用实验结果表明:酶液对果皮粉中半纤维素的降解随反应时间的延长降解率增加,反应5h的降解率就达到了62.44%。动物实验结果表明:实验组大鼠的采食量比对照组要高,差异极显著(P<0.01),日增重比对照组要高,差异极显著(P<0.01);实验组大鼠对饲料中半纤维素降解率数值与对照组相比没有差异显著;与对照组相比,实验组大鼠空肠部位的总菌数有显著差异(P<0.01),盲肠部位的总菌数没有显著差异,结肠中总菌数要显著低于对照组(P<0.01)。因此,木聚糖酶有利于果皮的脱皮,能促进大鼠的生长,改善肠道微生态平衡。

关键词: 白腐真菌, 木聚糖酶, 应用

Abstract:

Xylanase has a promising potential of applications in the feed industry as well as the food and pulp industry. These
applications of xylanase from Pleurotus ostreatus SYJ-042 were investigated. The results showed that the degradation of
hemicellulose in pear pericarp was gradually increased with reaction time. The degradation rate at 5 h reached 62.44%. Animal
experiments showed that the experimental group fed on xylanase-containing wheat diet had a higher food intake than the control
group and the difference was significant. The daily weight gain was also higher than that of the control group and a significant
difference was observed (P < 0.01). However, the dietary hemicellulose degradation rate in the experimental group compared
with the control group did not differ significantly. Administration of xylanase-containing diet also decreased the total bacterial
count in the jejunum and colon (P < 0.01) without resulting in a significant difference for the cecum (P < 0.01). In conclusion,
the xylanase was beneficial to remove the pericarp, promote the growth of rats, and maintain intestinal microecological balance.

Key words: white-rot fungi, xylanase, application

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