关键词: 食品, 肠炎沙门氏菌, 脉冲场凝胶电泳, 分子分型

Abstract:

Objective: To explore the relationship among Salmonella enteritidis strains isolated from imported and
exported foods in Shenzhen during 2005–2011, and develop a DNA fingerprint database of Salmonella enteritidis.
Methods: Chromosomal DNAs from 58 isolates in agarose were digested with the restriction enzyme Xba Ⅰ, and then
were analyzed by pulsed-field gel electrophoresis (PFGE). PFGE patterns were clustered using BioNumerics software.
Results: DNA fingerprint database of imported and exported foods in Shenzhen was successfully developed. The
results showed that there were 18 distractive PFGE patterns including 2 clusters. In cluster 1, Salmonella enteritidis
strains isolated from American poultry meat products had 91.45% similarity, and pattern 2 was the dominant type. In
cluster 2, 38 Salmonella enteritidis strains isolated from Chinese and Argentinian poultry meat products had 91.03%
similarity and its dominant type was P8. Conclusion: There were two closely correlated pandemic clones of Salmonella
enteritidis isolated from imported and exported foods. The development of DNA fingerprint database of imported and
exported foods can contribute to develop molecular subtyping surveillance network for active surveillance and source
tracking of food-borne pathogenic bacteria.

Key words: Salmonella enteritidis, pulsed-field gel electrophoresis, molecular subtyping