食品科学

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响应面法优化灰树花发酵全液多糖提取工艺及其抗肿瘤活性

张桂春,刘玉静,解卫海,辛晓林,周菊华   

  1. 鲁东大学生命科学学院,鲁东大学肿瘤免疫新技术研究中心,山东 烟台 264025
  • 出版日期:2013-12-25 发布日期:2014-01-03

Optimization of Microwave-Assisted Extraction of Polysaccharides from the Fermentation Broth of Crifola frondosa by Response Surface Methodology and Antitumor Activity of the Extracted Polysaccharides

ZHANG Gui-chun,LIU Yu-jing,XIE Wei-hai,XIN Xiao-lin,ZHOU Ju-hua   

  1. Institute for Tumor Immunology, College of Life Science, Ludong University, Yantai 264025, China
  • Online:2013-12-25 Published:2014-01-03

摘要:

研究高效提取灰树花发酵全液多糖的工艺及其抗肿瘤活性。以新鲜的灰树花发酵全液为材料,采用不同溶液pH值、微波功率、微波处理时间、热水浸提时间的单因素试验和响应面法优化微波辅助提取灰树花发酵全液多糖工艺;通过建立荷瘤小鼠模型,对灰树花发酵全液多糖的抗肿瘤活性进行研究。结果表明:微波辅助提取灰树花发酵全液多糖的最佳工艺条件为溶液pH7.0、微波功率570W、微波时间4.5min、热水浸提时间2h。此工艺条件下,灰树花发酵全液多糖得率为7.19g/L,比优化前提取效率提高24.39%。体外抗肿瘤实验表明,灰树花发酵全液多糖对小鼠S180肉瘤的抑制率为26.62%~63.01%,并可显著提高小鼠的胸腺指数和脾指数。

关键词: 响应面法, 灰树花, 发酵全液, 多糖, 微波辅助提取, 抗肿瘤

Abstract:

The microwave-assisted extraction of polysaccharides from the submerged fermentation broth of Grifola
frondosa and the anti-tumor activity of the extracted polysaccharides was evaluated in a tumor-bearing mouse model.
Different extractions parameters including solvent pH, microwave power, treatment time and extraction time were optimized
using response surface methodology. The optimum extraction conditions that provided maximum yield of polysaccharides
of 7.19 g/L, which was 24.39% higher than before the optimization were found as follows: solvent pH of 7.0, microwave
power of 570 W, treatment time of 4.5 min and hot water extraction time of 2 h. Furthermore, the extracted polysaccharides
not only had anti-tumor activity against mouse S180 sarcoma cells as indicated by its inhibition on the S180 sarcoma cellderived
tumor growth by 26.62%–63.01%, but also significantly increased both thymus index and spleen index in mice.

Key words: response surface methodology, Crifola frondosa, fermentation broth, polysaccharide, microwave-assisted extraction, anti-tumor activity