关键词: 超声辅助乳化微萃取, 低密度有机溶剂, 气相色谱-质谱法, 芳香水, 草豆蔻

Abstract:

A novel method using a polyethylene Pasteur pipette, ultrasound-assisted emulsification microextraction
(USAEME) with low density organic solvent (n-hexane) was successfully developed for the analysis of chemical constituents
in aromatic water from Alpinia katsumadai Hayata by gas chromatography-mass spectrometry (GC-MS). Factors affecting
the extraction process, such as the type and volume of extraction solvents and extraction time, were optimized. The optimum
extraction conditions were found as follows: extraction of 1 mL of aromatic water with 80 μL of n-hexane for 10 min.
Meanwhile, 52 volatile compounds were identified in this study, with eucalyptol (28.06%), 3-phenyl-2-butanone (22.69%),
methyl cinnamate (9.34%), α-terpineol (7.51%), linalool (4.48%), borneol (4.11%), 4-terpineol (3.40%) and myrtenol
(3.77%) being the major volatile compounds in the aromatic water. The USAEME technique proved to be a simple, efficient,
cheap and environment-friendly method for the determination of chemical components in the aromatic water.

Key words: ultrasound-assisted emulsification microextraction (USAEME), low density organic solvent, gas chromatography-mass spectrometry (GC-MS), aromatic water, Alpinia katsumadai Hayata