关键词: 超声波萃取法, 凝胶渗透色谱法, 气相色谱电子捕获检测法, 鲍鱼不同组织, 多氯联苯

Abstract:

A simple, efficient, and sensitive method with low matrix interference for determination of 7 polychlorinated
biphenyls (PCBs) in 3 different tissues of abalone using gas chromatography-electron capture detector (GC-ECD) has been
developed. Muscle tissue samples were treated by ultrasonic extraction with n-hexane and gel permeation chromatography
(GPC). Further purification on a florisil solid phase extraction (SPE) column was needed for both viscera tissue and whole
cowry samples before analysis. The linearity of the method ranged from 1.25 to 100 μg/L, with correlation coefficients
ranging from 0.9990 to 0.9999. The limits of detection for 7 PCBs were 0.04–0.06 μg/kg. The recoveries of all the PCBs
from muscle, viscera and whole cowry samples spiked at different concentrations were 80.9%–105%, 71.2%–92.3%,
and 72.6%–91.4%, respectively, with relative standard deviations of 3.22%–6.80%, 3.46%–7.01%, and 3.83%–6.86% (n = 5),
respectively. This simple and rapid method had the advantages of low matrix interference, wide linear range and high sensitivity,
accuracy and precision and thus could meet the requirements for the analysis of PCBs in different tissues of abalone.

Key words: ultrasonic extraction, gel permeation chromatography (GPC), gas chromatography with electron capture detector (GC-ECD), different tissues of abalone, polychlorinated biphenyls (PCBs)