食品科学 ›› 2014, Vol. 35 ›› Issue (1): 204-208.doi: 10.7506/spkx1002-6630-201401040

• 营养卫生 • 上一篇    下一篇

黑灵芝多糖对S-180荷瘤小鼠腹腔巨噬细胞cAMP/PKA、IP3 /Ca2+及DAG/PKC信号通路的影响

黄建琴,聂少平*,张莘莘,黄丹菲,朱科学,谢明勇   

  1. 南昌大学 食品科学与技术国家重点实验室,江西 南昌 330047
  • 收稿日期:2013-07-16 修回日期:2013-12-10 出版日期:2014-01-15 发布日期:2014-01-22
  • 通讯作者: 聂少平 E-mail:spnie@ncu.edu.cn
  • 基金资助:

    国家自然科学基金重点项目(31130041);国家自然科学基金项目(31071532);
    “十二五”国家科技支撑计划项目(2012BAD33B06);教育部“新世纪优秀人才支持计划”项目(NCET-12-0749)

Effect of a Polysaccharide from Ganoderma atrum on cAMP/PKA, IP3/Ca2+ and DAG/PKC Signaling Pathways in Peritoneal Macrophages of S-180 Tumor-Bearing Mice

HUANG Jian-qin, NIE Shao-ping*, ZHANG Shen-shen, HUANG Dan-fei, ZHU Ke-xue, XIE Ming-yong   

  1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China
  • Received:2013-07-16 Revised:2013-12-10 Online:2014-01-15 Published:2014-01-22
  • Contact: Shao-Ping NIE E-mail:spnie@ncu.edu.cn

摘要:

目的:探讨黑灵芝多糖(polysaccharide from Ganoderma atrum,PSG-1)对S-180荷瘤小鼠腹腔巨噬细胞环磷酸腺苷(cyclic adenosine monophosphate,cAMP)/细胞蛋白激酶A(protein kinase A,PKA)、三磷酸肌醇(inositol triphosphate,IP3)/Ca2+及甘油二酯(diacylglycerol,DAG)/蛋白激酶C(protein kinase C,PKC)信号通路的影响。方法:将S-180细胞接种于BALB/c小鼠体内建立荷瘤小鼠模型,成模后收集腹腔巨噬细胞进行体外培养,用不同浓度的PSG-1干预;ELISA法分别检测巨噬细胞培养上清液中的IP3、DAG和AMP含量;流式细胞仪测定细胞内Ca2+含量;Western blotting测定细胞PKA及PKC蛋白表达。结果:PSG-1在20~160 μg/mL质量浓度范围能促进S-180荷瘤小鼠腹腔巨噬细胞产生cAMP、IP3和DAG,同时能增加细胞内Ca2+含量,并显著增强PKA及PKC蛋白表达量。结论:PSG-1能有效激活S-180荷瘤小鼠腹腔巨噬细胞cAMP/PKA、IP3/Ca2+及DAG/PKC信号通路。由此推测,PSG-1可能通过cAMP/PKA、IP3/Ca2+及DAG/PKC信号通路促进S-180荷瘤小鼠腹腔巨噬细胞抗肿瘤作用。

关键词: 黑灵芝多糖, 腹腔巨噬细胞, 抗肿瘤, 信号通路

Abstract:

Objective: To explore the effects of a polysaccharide from Ganoderma atrum (PSG-1) on cyclic adenosine
monophosphate (cAMP)/protein kinase A (PKA), inositol triphosphate (IP3)/Ca2+ and diacylglycerol (DAG)/protein kinase C
(PKC) signaling pathways in peritoneal macrophages of S-180 tumor-bearing mice. Methods: A tumor-bearing mouse model
was established by inoculating mouse sarcoma S-180 cells into BALB/c mice; peritoneal macrophages were collected from
the S-180 tumor-bearing mice, then cultured in vitro and treated with PSG-1 at various concentrations. The IP3, DAG and
cAMP levels in cell culture supernatant were measured by ELISA. The intracellular Ca2+ content was assayed by a flow
cytometric method. The PKA and PKC protein expression in macrophages was determined by Western blotting. Results:
PSG-1 in the concentration range of 20–160 μg/mL stimulated the production of IP3, DAG and cAMP in the macrophages
from S-180 tumor-bearing mice, increased the intracellular Ca2+ content, and increased the protein expression of PKA and
PKC. Conclusion: PSG-1 can exert anti-tumor activity in peritoneal macrophages of S-180 tumor-bearing mice through the
activation of cAMP/PKA, IP3/Ca2+ and DAG/PKC signaling pathways.

Key words: polysaccharides from Ganoderma atrum, peritoneal macrophages, anti-tumor activity, signaling pathway

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