湿法消解-原子荧光光谱法测定湘葛一号中的砷、汞、铅

doi:10.7506/spkx1002-6630-201404031

食品科学 ›› 2014, Vol. 35 ›› Issue (4): 151-154.doi: 10.7506/spkx1002-6630-201404031

湿法消解-原子荧光光谱法测定湘葛一号中的砷、汞、铅

叶惠煊1，谭舟2，刘向前1,*，周斌文2，熊力夫3

1.湖南中医药大学药学院，湖南省中药现代化研究重点实验室，湖南长沙 410208；
2.长沙市雨花区日成晟食品添加剂厂，湖南长沙 410014；3.长沙市葛根工程技术研究中心，湖南长沙 410000

收稿日期:2013-09-18 修回日期:2014-01-16 出版日期:2014-02-25 发布日期:2014-03-17
通讯作者: 刘向前 E-mail:lxq0001cn@163.com
基金资助:
湖南省自然科学基金重点项目（11JJ2042）；长沙市科技局科技计划重点项目（K1207010-21）；
湖南中医药大学药物分析学“十二五”校级重点学科建设项目

Determination of Arsenic, Mercury and Lead in Radix Puerariae by Atomic Florescence Spectrophotometry with Wet Digestion

YE Hui-xuan1, TAN Zhou2, LIU Xiang-qian1,*, ZHOU Bin-wen2, XIONG Li-fu3

1. Modernization Laboratory of Traditional Chinese Medicine and Drug, College of Pharmacy, Hunan University of Traditional
Chinese Medicine, Changsha 410208, China; 2. Ri Cheng Sheng Food Additive Factory in Yuhua District of Changsha,
Changsha 410014, China; 3. Pueraria Engineering Research Center, Changsha 410000, China

Received:2013-09-18 Revised:2014-01-16 Online:2014-02-25 Published:2014-03-17
Contact: Xiang-Qian LIU E-mail:lxq0001cn@163.com

摘要/Abstract

摘要：

3 批不同采收期湘葛一号样品经硝酸-高氯酸的混合液湿法消解后，用原子荧光光谱法测定As、Hg和Pb的含量。As、Hg和Pb的线性范围分别为0～16.0、0～5.0 ng/mL和0～40.0 ng/mL；相关系数r分别为0.999 9、0.999 0和0.999 7；检出限分别为0.050、0.012 μg/L和0.038 μg/L（n=11）；样品平均回收率分别为98.73%、101.22%和99.43%（n=6）。该法方法简便、准确、精密度好，可用于湘葛一号中As、Hg和Pb的含量的快速测定。

关键词: 湿法消解, 原子荧光光谱法, 湘葛一号, 砷, 汞, 铅

Abstract:

A method for determination of the contents of heavy metals including As, Hg and Pb in 3 batches of Radix
Puerariae (Pueraria thomsonii Benth cultivar “Xiangge No.1” grown in Hunan province harvested at different times) was
developed using atomic fluorescence spectrophotometry in combination with wet digestion. Samples were wet digested by
nitric acid and perchloric acid and then detected by hydride generation atomic florescence spectrophotometry. The linear
ranges for As, Hg and Pb were 0－16.0, 0－5.0 and 0－40.0 ng/mL, respectively, with correlation coefficients of 0.999 9,
0.999 0 and 0.999 7, respectively. Their limits of detection were 0.050, 0.012 and 0.038 μg/L (n = 11), respectively. Their
recovery rates were 98.73%, 101.22% and 99.43% (n = 6), respectively. This method allows simple, fast and accurate
determination of As, Hg and Pb in Radix Puerariae.

Key words: wet digestion, atomic fluorescence spectrophotometry, Pueraria thomsonii Benth cultivar “Xiangge No.1”, As, Hg, Pb

中图分类号:

R284.1

叶惠煊1，谭舟2，刘向前1,*，周斌文2，熊力夫3. 湿法消解-原子荧光光谱法测定湘葛一号中的砷、汞、铅[J]. 食品科学, 2014, 35(4): 151-154.

YE Hui-xuan1, TAN Zhou2, LIU Xiang-qian1,*, ZHOU Bin-wen2, XIONG Li-fu3. Determination of Arsenic, Mercury and Lead in Radix Puerariae by Atomic Florescence Spectrophotometry with Wet Digestion[J]. FOOD SCIENCE, 2014, 35(4): 151-154.

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湿法消解-原子荧光光谱法测定湘葛一号中的砷、汞、铅

Determination of Arsenic, Mercury and Lead in Radix Puerariae by Atomic Florescence Spectrophotometry with Wet Digestion

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