关键词: 莲藕, 提取, 结合态阿魏酸, 比色法, 响应面法优化

Abstract:

In this work, bound ferulic acid (FA) was extracted from lotus root with NaOH solution following the removal
of free FA with 75% ethanol solution. FA content was spectrometrically determined at 324 nm, where it had characteristic
absorption. A preliminary investigation into four extraction parameters including NaOH concentration, temperature,
extraction time and solvent-to-solid ratio was carried out by one-factor-at-a-time design. By using Box-Behnken design and
response surface analysis, the optimal extraction conditions were established as follows: NaOH concentration,1.49 mol/L;
extraction time, 2.66 h; extraction temperature, 84.45 ℃, and solvent-to-solid ratio, 5.5:1 (mL/g). Under the optimized
conditions, the yield of found FA from lotus root was 0.217 mg/g (by dry weight).

Key words: lotus root, extraction, bound ferulic acid, colorimetric method, response surface methodology (RSM)