食品科学

• 基础研究 • 上一篇    下一篇

金荞麦(-)-表儿茶素抗氧化活性研究

黄仁术,易 凡,何惠利,吴宋华亲,方娇娇,杨 敏   

  1. 1.植物细胞工程安徽省工程技术研究中心,安徽 六安 237012;2.皖西学院生物与制药工程学院,安徽 六安 237012
  • 出版日期:2014-08-15 发布日期:2014-08-25

Antioxidant Activity of (-)-Epicatechin from the Root Tubers of Fagopyrum dibotrys

HUANG Ren-shu, YI Fan, HE Hui-li, WU Song-hua-qin, FANG Jiao-jiao, YANG Min   

  1. 1. Engineering Technology Research Center of Plant Cell Engineering, Anhui Province, Lu’an 237012, China;
    2. College of Biological and Pharmaceutical Engineering, West Anhui University, Lu’an 237012, China
  • Online:2014-08-15 Published:2014-08-25

摘要:

研究金荞麦中(-)-表儿茶素活性物质的抗氧化活性。超氧阴离子自由基清除实验表明其抗氧化率达到83.08%;羟自由基清除实验表明活性物质质量浓度对清除率的影响差异极显著(P<0.01),质量浓度为0.350 mg/mL时清除率达77.30%;还原力测定实验表明其还原力达到同质量浓度VC的48.02%,且质量浓度对还原力的影响差异极显著(P<0.01);乙醇提取物中活性物质含量达1.40 mg/mL,占块根干物质质量的2.52%。实验证明金荞麦(-)-表儿茶素活性物质具有很好的抗氧化活性,可作为一种天然、高效的抗氧化剂。

关键词: 金荞麦, (-)-表儿茶素, 抗氧化

Abstract:

The antioxidant activity of (-)-epicatechin extracted with ethanol from the root tubers of Fagopyrum dibotrys
was evaluated in this study. Results showed that the (-)-epicatechin extract could scavenge 83.08% of superoxide anion
radical; its hydroxyl radical scavenging capacity was very significantly affected by its concentration (P < 0.01), and
77.30% of hydroxyl radical was scavenged at a concentration of 0.350 mg/mL. The epicatechi extract, showing a significant
concentration-effect relationship (P < 0.01), was only 48.02% as effective as vitamin C at the same concentration in
reducing Fe3+. The ethanol extract contained 1.40 mg/mL active components, accounting for 2.52% of the dried root tubers
of Fagopyrum dibotrys. This study proves that (-)-epicatechin from the root tubers of Fagopyrum dibotrys has strong
antioxidant activity as a natural potent antioxidant.

Key words: Fagopyrum dibotrys, (-)-epicatechin, antioxidant

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