食品科学

• 工艺技术 • 上一篇    下一篇

离子沉淀法分离八月瓜果皮中的总多酚

欧阳玉祝,李雪峰,姚懿桓   

  1. 吉首大学食品科学研究所,湖南 吉首 416000
  • 出版日期:2014-08-25 发布日期:2014-08-25
  • 基金资助:

    吉首大学植物资源保护与利用湖南省高校重点实验室开放基金项目(13jdzb094);
    湖南湘西自治州科技计划项目(州财企函[2013]7号)

Separation of Total Polyphenols from Akebia trifoliate Peel by Calcium Precipitation Method

OUYANG Yu-zhu, LI Xue-feng, YAO Yi-huan   

  1. Institute of Food Science, Jishou University, Jishou 416000, China
  • Online:2014-08-25 Published:2014-08-25

摘要:

以八月瓜果皮干粉为原料分离总多酚,在单因素试验基础上,采用响应面法优化钙沉淀分离总多酚的工艺条件,考察沉淀剂用量、沉淀温度和时间对总多酚提取率的影响。结果表明,钙沉淀分离总多酚的最佳工艺条件为:在50 mL提取液中,添加90 mL饱和石灰水,70 ℃沉淀38 min。总多酚提取率为5.103 7%。试验模型的预测值与组合实验结果吻合,分离产品总多酚纯度为84.417 4%。

关键词: 八月瓜, 总多酚, 响应面法, 钙沉淀

Abstract:

A calcium precipitation method to separate total polyphenols from a 70% ethanol extract of dried powdered
Akebia trifoliate peel was proposed and optimized by response surface methodology. The yield of total polyphenols was
investigated as a function of precipitator dosage, precipitation temperature and time. The results showed that the optimum
precipitation conditions that provided maximum total polyphenol yield of 5.103 7% were obtained when 50 mL of the extract
was precipitated by adding 90 mL of clear saturated limewater at 70 ℃ for 38 min. There was a good agreement between the
predicted and experimental data for total polyphenol yield. The separated precipitate contained 84.417 4% total polyphenols.

Key words: Akebia trifoliate, total polyphenols, response surface methodology, calcium precipitation

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