关键词: 液相色谱-质谱, 同时测定, 水产品, 兽药

Abstract:

A method for the simultaneous determination of 78 veterinary drugs in aquatic products (fish, shrimp and
shellfish) was established using liquid chromatography tandem mass spectrometry (LC-MS-MS). Effects of experimental
conditions such as extraction solvents and Na2EDTA adding on extraction efficiency were investigated. Under the optimized
conditions, recoveries of 78 target drugs calculated from matrix match calibration curve at spiked level of 4 and 20 μg/kg
were 40.8%–125.6% for fish, 42.9%–131.9% for shrimp and 43.5%–136.4% for shellfish with RSD (n = 4) of 0.1%–16.0%,
0.1%–14.0% and 0.3%–15.8%, respectively. The detection limits drugs ranged from 0.1 to 0.5 μg/kg. The method is
sensitive, accurate and fast and can be successfully applied in the analysis of residual veterinary drugs in shrimp samples
from the aquatic farming areas of Zhangzhou, Fujian province.

Key words: liquid chromatography tandem mass spectrometry (LC-MS-MS), simultaneous determination, aquatic products, veterinary drugs