多不饱和脂肪酸对大鼠肠道菌群及脂肪代谢相关基因的影响

doi:10.7506/spkx1002-6630-201417044

摘要/Abstract

摘要：

目的：研究多不饱和脂肪酸（polyunsaturated fat acids，PUFA）饮食对大鼠肠道菌群及相关脂肪因子的影响，探讨其作用机理。方法：将30 只5 周龄健康大鼠分为正常对照组、n-6 PUFA组和n-3 PUFA组，自由摄食饮水8 周，每周记录大鼠体质量，实验结束时取大鼠盲肠粪便和肝脏，用实时荧光定量聚合酶链式反应检测双歧杆菌（Bifidobacterium，Bif）、乳酸杆菌（Lactobacillus，Lac）、肠道细菌Akkermansia muciniphila（Akk）和脂肪因子脂肪酸合成酶（fatty acid synthetase，FAS）、禁食诱导脂肪因子（fasting-induced adipose factor，FIAF）的水平；并将大鼠盲肠石蜡包埋、切片、苏木精-伊红染色测量其黏膜厚度。结果：n-3 PUFA组与正常对照组相比，肥胖程度和FAS水平明显降低（P＜0.05），Bif、Lac、Akk和FIAF水平明显升高（P＜0.05），肠道黏膜厚度明显增加；n-6 PUFA组大鼠的各项指标和正常对照组相比均无统计学差异。结论：n-3 PUFA饮食与n-6 PUFA饮食相比，可改变肠道菌群，抑制肥胖的发生和发展。

关键词: n-3多不饱和脂肪酸, 肥胖, Akkermansia muciniphila, 肠道黏膜

Abstract:

Purpose: To determine the effect of PUFA diet on gut microbiota and obesity-related cell factors in rats. Methods:
A total of 30 five-week-old healthy female rats were randomly divided into three groups: control group, n-6 PUFAcontaining
diet group and n-3 PUFA-containing diet group. The rats had free access to the feeds and water for eight weeks.
Body weight was recorded once a week during the trail period and the levels of Bifidobacterium (Bif), Lactobacillus (Lac),
Akkermansia muciniphila (Akk) and obesity-related cell factors, fatty acid synthetase (FAS) and fasting-induced adipose
factor (FIAF) were evaluated by qRT-PCR at the end of the trial. Results: The adiposity and FAS level in the n-3 PUFAcontaining
diet group were significantly lower than that in the control group (P ＜ 0.05), and the levels of Bif, Lac, Akk and
FIAF, and the thickness of the intestinal mucosa in the n-3 PUFA-containing diet model group were significantly higher than
those in the control group (P ＜ 0.05). Conclusion: An obvious change in gut microbiota was observed in rats fed n-3 PUFAcontaining
diet; meanwhile the occurrence and development of adiposity were inhibited.

Key words: n-3 PUFA, obesity, Akkermansia muciniphila, intestinal mucosa

中图分类号:

R378

乔立君，郑征，马文慧，芦庭秀，盖晓英，葛银林. 多不饱和脂肪酸对大鼠肠道菌群及脂肪代谢相关基因的影响[J]. 食品科学, doi: 10.7506/spkx1002-6630-201417044.

QIAO Li-jun, ZHENG Zheng, MA Wen-hui, LU Ting-xiu, GAI Xiao-ying, GE Yin-lin. Effect of PUFA-Containing Diet on Gut Microbiota and Fat Metabolism-Related Genes in Rats[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201417044.

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多不饱和脂肪酸对大鼠肠道菌群及脂肪代谢相关基因的影响

Effect of PUFA-Containing Diet on Gut Microbiota and Fat Metabolism-Related Genes in Rats

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