食品科学

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花椒挥发油的提取工艺优化及抗肿瘤活性分析

韩胜男,李 妍,张晓杭,蒋建兰*   

  1. 天津大学化工学院,天津 300072
  • 出版日期:2014-09-25 发布日期:2014-09-17
  • 通讯作者: 蒋建兰
  • 基金资助:

    国家自然科学基金青年科学基金项目(81102900)

Extraction and Antitumor Activity of Essential Oil from Zanthoxylum bungeanum Seeds

HAN Sheng-nan, LI Yan, ZHANG Xiao-hang, JIANG Jian-lan*   

  1. School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
  • Online:2014-09-25 Published:2014-09-17
  • Contact: JIANG Jian-lan

摘要:

采用水蒸气蒸馏法提取花椒中的挥发油,以得油率为评价指标,以料液比、冷浸时间和提取时间为考察因素,通过星点设计-响应面法优选花椒挥发油提取工艺。结果表明:花椒挥发油水蒸气蒸馏提取的最佳工艺条件为料液比1∶12(g/mL)、冷浸时间2.5 h、提取时间4.7 h,得油率可达9.284%。采用MTT法检测花椒挥发油的体外抗肿瘤活性,测得花椒挥发油对HeLa、A549、K562 三种细胞的IC50值分别为(11.2±0.2)、(6.26±0.05)、(1.37±0.03) mg/mL。表明花椒挥发油具有较强的体外抗肿瘤活性,且对3 种肿瘤细胞的生长均有抑制作用。

关键词: 花椒, 挥发油, 水蒸气蒸馏提取, 抗肿瘤

Abstract:

Response surface methodology was used to optimize the steam distillation extraction of essential oil from
Zanthoxylum bungeanum seeds. The yield of essential oil was investigated with respect to solid-to-solvent ratio, cold water
soaking time and extraction time. The optimum extraction conditions were determined as follows: solid-to-solvent ratio 1:12
(g/mL), cold water soaking time 2.5 h and extraction time 4.7 h, leading to an extraction yield of 9.284%. MTT assay was
used to detect the antitumor activity of the essential oil. Its 50% inhibition concentration (IC50) for HeLa, A549 and K562
cells were (11.2±0.2), (6.26±0.05) and (1.37±0.03) mg/mL, respectively, suggesting potent antitumor activity in vitro and
inhibitory effect on various cancer cells.

Key words: Zanthoxylum bungeanum, essential oil, steam distillation, antitumor activity

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