食品科学

• 分析检测 • 上一篇    下一篇

高效液相色谱串联四极杆质谱法同时测定豆芽中7 种药物残留

胡祥娜1,顾亚萍1,*,林慧纯1,王 瑞1,方长发2   

  1. 1.深圳市农产品质量安全检验检测中心,广东 深圳 518040;
    2.深圳职业技术学院应用化学与生物技术学院,广东 深圳 518055
  • 出版日期:2014-10-25 发布日期:2014-11-07

Simultaneous Determination of Seven Drug Residues in Bean Sprouts by High Performance Liquid Chromatography-Tandem Mass Spectrometry

HU Xiang-na1, GU Ya-ping1,*, LIN Hui-chun1, WANG Rui1, FANG Chang-fa2   

  1. 1. Shenzhen Centre of Inspection and Testing for Agricultural Products, Shenzhen 518040, China;
    2. School of Applied Chemistry and Biological Technology, Shenzhen Polytechnic, Shenzhen 518055, China
  • Online:2014-10-25 Published:2014-11-07

摘要:

为加强对豆芽类产品的监管,研究豆芽中7 种药物(6-苄基腺嘌呤、4-氯苯氧乙酸、2,4-D、赤霉素、多菌灵、腐霉利和恩诺沙星)同时检测的方法。该方法使用乙腈对豆芽中7 种药物进行同时提取,使用高效液相串联四极杆质谱仪对上述7 种药物进行检测。7 种药物在0.01~10 μg/mL(多菌灵和恩诺沙星在0.01~1 μg/mL)内线性良好(R2≥0.987)。两种豆芽中7 种药物的3 个添加量(分别为0.002、0.2、0.5 mg/kg)回收实验结果表明:7 种药物的回收率为60.31%~108.51%,相对标准偏差为1.03%~13.10%,方法检出限为0.010 5~0.125 μg/kg。

关键词: 高效液相色谱串联四极杆质谱仪, 豆芽, 6-苄基腺嘌呤, 4-氯苯氧乙酸, 2, 4-D, 赤霉素, 多菌灵, 腐霉利, 恩诺沙星

Abstract:

A high performance liquid chromatography-tandem mass spectrometry (HPLC-MS-MS) method was developed
for the simultaneous determination of seven drug residues including 6-benzylaminopurine, para-chlorophenoxyacetic acid,
2,4-D, gibberellin A3, carbendazim, procymidone, and enrofloxacin in bean sprouts. The seven compounds from samples
were simultaneously extracted with acetonitrile and analyzed by HPLC-MS-MS. The calibration curves showed good
linearity in the range of 0.01–10 μg/mL for 6-benzylaminopurine, para-chlorophenoxyacetic acid, 10-benzylaminopurine,
gibberellin A3, and procymidone, and in the range of 0.01–1 μg/mL for carbendazim and enrofloxacin with correlation
coefficients more than or equal to 0.987. The recoveries of these drug residues at three spiked levels (0.002, 0.2 and
0.5 mg/kg) ranged from 60.31% to 108.51%, with relative standard deviation (RSDs) between 1.03% and 13.10% and
method detection limit (MDL) between 0.010 5 and 0.125 μg/kg.

Key words: high performance liquid chromatography tandem mass spectrometry (HPLC-MS-MS), bean sprouts;
6-benzylaminopurine,
para-chlorophenoxyacetic acid, 2,4-D, gibberellin A3, carbendazim , procymidone, enrofloxacin

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