食品科学

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富硒绿茶对大鼠非酒精性脂肪肝Ca2+-ATPase活性和表达的影响

王凤杰,陈显兵,张书毓,谭志鑫,向国敏,刘锦红   

  1. 1.湖北民族学院 生物资源保护与利用湖北省重点实验室,湖北 恩施 445000;
    2.湖北民族学院科技学院,湖北 恩施 445000
  • 出版日期:2014-11-15 发布日期:2014-11-06

Effect of Selenium-Enriched Green Tea on Activity and Expression of Sarco(endo)Plasmic Reticulum Ca2+-ATPase (SERCA) in Rats with Nonalcoholic Fatty Liver Disease (NAFLD)

WANG Feng-jie, CHEN Xian-bing, ZHANG Shu-yu, TAN Zhi-xin, XIANG Guo-min, LIU Jin-hong   

  1. 1. Key Laboratory of Biologic Resources Protection and Utilization of Hubei Province, Hubei Minzu University, Enshi 445000, China;
    2. Science and Technology College of Hubei Minzu University, Enshi 445000, China
  • Online:2014-11-15 Published:2014-11-06

摘要:

目的:研究恩施富硒绿茶对大鼠非酒精性脂肪肝病(nonalcoholic fatty liver disease,NAFLD)中肝脏肌浆网/内质网Ca2+-ATPase(sarco(endo)plasmic reticulum Ca2+-ATPase,SERCA)活性及其表达的影响,探讨富硒绿茶防治NAFLD的可能作用机制。方法:将32 只Wistar大鼠分成4 组,分别饲喂标准饲料+常规饮水(NC组)、高脂饲料+常规饮水(HC组)、高脂饲料+普通绿茶(TC组)、高脂饲料+富硒绿茶(SeC组)。9 周后处死,观察大鼠一般情况,测定肝细胞内Ca2+含量、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)水平、肝脏组织ATPase活性;采用免疫组化染色和Western blotting检测肝脏内总SERCA蛋白表达,观察各指标变化情况。结果:HC组大鼠肝细胞内Ca2+含量明显增高,Na+-K+-ATPase、Ca2+-ATPase活性降低,SERCA蛋白表达降低;富硒绿茶干预后Ca2+含量降低,酶活性升高,表达增加,较普通绿茶效果明显。结论:富硒绿茶对高脂饮食诱导的大鼠非酒精性脂肪肝病具有干预作用,可能是通过降低肝细胞内Ca2+和TNF-α含量而改善肝脏能量代谢障碍,同时提高了SERCA活性,增强其蛋白表达而实现的。

关键词: 富硒绿茶, 非酒精性脂肪肝病, 肌浆网/内质网Ca2+-ATPase

Abstract:

Objective: To investigate the effect of selenium-enriched green tea on the activity and expression of sarco(endo)
plasmic reticulum Ca2+-ATPase (SERCA) on model rats with experimental non-alcoholic fatty liver disease (NAFLD) and
explore the possible mechanisms for the prevention and treatment of NAFLD. Methods: Totally 32 Wistar rats were divided
into 4 groups randomly and fed with standard diet and water (NC), high-fat diet and water (HC), high-fat diet and tea (TC),
high-fat diet and selenium-enriched tea (SeC), respectively. After 9 weeks, the rats were killed to investigate the changes in
Na+-K+-ATPase and Ca2+-ATPase activities. Meanwhile, hepatic tumor necrosis factor-α (TNF-α) and Ca2+ levels and the
protein expression of SERCA in hepatocytes was evaluated by immunohistochemistry (IHC) and Western blotting. Results:
The protein expression of SERCA and the activities of Na+-K+-ATPase and Ca2+-ATPase were significantly decreased and
the Ca2+ level was remarkably increased in HC group. The activity and expression of Na+-K+-ATPase and Ca2+-ATPase in
liver were markedly upregulated in intervention group. On the contrary, the level of Ca2+ in SeC group was greatly reduced
when compared with ordinary tea. Conclusion: Selenium-enriched green tea can improve hepatic energy metabolism
disorder, protect liver function, and prevent liver damage in NAFLD rats.

Key words: selenium-enriched green tea, nonalcoholic fatty liver disease (NAFLD), sarco(endo)plasmic reticulum

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