甘肃河西走廊葡萄酒产区高产β-葡萄糖苷酶酵母菌株筛选

doi:10.7506/spkx1002-6630-201423028

食品科学

甘肃河西走廊葡萄酒产区高产β-葡萄糖苷酶酵母菌株筛选

侯晓瑞1,2,3，王婧1,2,3，杨学山4，盛文军1,2,3，祝霞1,2,3，韩舜愈1,2,3,*

1.甘肃农业大学食品科学与工程学院，甘肃兰州 730070；2.甘肃省葡萄与葡萄酒工程学重点实验室，甘肃兰州 730070；
3.甘肃省葡萄酒产业技术研发中心，甘肃兰州 730070；4.甘肃农业大学生命科学技术学院，甘肃兰州 730070

发布日期:2014-12-15

Screening of Yeast Strains Producing β-Glucosidase from Hexi Corridor Wine-Producing Regions of Gansu Province

HOU Xiao-rui1,2,3, WANG Jing1,2,3, YANG Xue-shan4, SHENG Wen-jun1,2,3, ZHU Xia1,2,3, HAN Shun-yu1,2,3,*

1. College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China;
2. Key Laboratory of Viticulture and Enology in Gansu Province, Lanzhou 730070, China;
3. Research and Development Center of Wine Industry Technology in Gansu Province, Lanzhou 730070, China;
4. College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China

Published:2014-12-15

摘要/Abstract

摘要：

以甘肃河西走廊葡萄酒产区成熟葡萄浆果自然发酵过程中分离得到的115 株酵母菌株为出发菌株，采用对硝基苯基-β-D-吡喃葡萄糖苷为底物的平板初筛，摇瓶复筛，对高产β-葡萄糖苷酶酵母菌株进行WL营养培养基和赖氨酸培养基初步分类以及26S rDNA D1/D2区序列分析。结果表明：6 株酵母β-葡萄糖苷酶活性与商业酵母ICV-D254酶活性相似，酶活力可达（51.40±4.74） mU/mL，其中菌株QLFE、MQFEH-1、MQFSC-3、MQFEH-2和MQFSM-3为酿酒酵母属，菌株QLFE-4为梅奇酵母属，与分子鉴定结果一致。

关键词: 酵母筛选, &beta, -葡萄糖苷酶活性, 鉴定, 26S rDNA D1/D2区序列分析

Abstract:

Totally 115 yeast strains isolated from spontaneously fermented ripe grape berries from Hexi Corridor wineproducing
regions of Gansu province were subjected to two-round screening on plates followed by in shaking flasks using
p-nitrophenyl-β-D-glucopyranoside (p-NPG) as a substrate for β-glucosidase activity. The strains with higher β-glucosidase
activity were subjected to taxonomic identification using WL nutrient medium and lysine medium, and sequence analysis of
the 26S rDNA D1/D2 domain. Six yeast isolates with β-glucosidase activity similar to that of commercial yeast ICV-D254,
(51.40±4.74) mU/mL, were obtained. Strains QLFE, MQFEH-1, MQFSC-3, MQFEH-2 and MQFSM-3 were identified as
Saccharomyces cerevisiae, and QLFE-4 as Metschnikowia. These identification results were in accordance with those from
sequence analysis of the 26S rDNA D1/D2 domain.

Key words: yeast screening, β-glucosidase activity, identification, sequence analysis of the 26S rDNA D1/D2 domain

中图分类号:

TS261.1

侯晓瑞1,2,3，王婧1,2,3，杨学山4，盛文军1,2,3，祝霞1,2,3，韩舜愈1,2,3,*. 甘肃河西走廊葡萄酒产区高产β-葡萄糖苷酶酵母菌株筛选[J]. 食品科学, doi: 10.7506/spkx1002-6630-201423028.

HOU Xiao-rui1,2,3, WANG Jing1,2,3, YANG Xue-shan4, SHENG Wen-jun1,2,3, ZHU Xia1,2,3, HAN Shun-yu1,2,3,*. Screening of Yeast Strains Producing β-Glucosidase from Hexi Corridor Wine-Producing Regions of Gansu Province[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201423028.

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甘肃河西走廊葡萄酒产区高产β-葡萄糖苷酶酵母菌株筛选

Screening of Yeast Strains Producing β-Glucosidase from Hexi Corridor Wine-Producing Regions of Gansu Province

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