食品科学

• 生物工程 • 上一篇    下一篇

自然发酵苹果渣中的微生物区系分析

孙文静1,陈义伦1,吴茂玉2,王凤忠3,黄坤勇4,朱宇竹1,周 波1,*   

  1. 1.山东农业大学食品科学与工程学院,山东 泰安 271018;2.中华全国供销合作总社济南果品研究院,山东 济南 250014;
    3.中国农业科学院农产品加工研究所,北京 100193;4.中宁牧光生物科技有限公司,宁夏 中卫 755100
  • 发布日期:2014-12-15

Analysis of Microflora Profile in Naturally Fermented Apple Pomace

SUN Wen-jing1, CHEN Yi-lun1, WU Mao-yu2, WANG Feng-zhong3, HUANG Kun-yong4, ZHU Yu-zhu1, ZHOU Bo1,*   

  1. 1. College of Food Science and Engineering, Shandong Agricultural University, Taian 271018, China;
    2. Jinan Fruit Research Institute, All China Federation of Supply and Marketing Cooperatives, Jinan 250014, China;
    3. Institute of Agro-products Processing Sciences and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    4. Biotechnology Corporation of Zhongning Mu-guang, Zhongwei 755100, China
  • Published:2014-12-15

摘要:

以自然发酵的苹果渣为对象,采用选择平板分离技术,对其不同层次的微生物区系组成进行分析。通过形态学观察、生理生化测定,结合ITS区、26S rRNA和16S rRNA基因序列分析等多相分类法对分离获得的微生物进行鉴定,研究苹果渣不同温度层中的微生物区系组成。结果表明:自然发酵苹果渣中主要微生物组成为酵母菌和乳酸菌,从28、37、45、50 ℃这4 个温度层中分离得到10 株菌,4 个温度层的优势菌分别鉴定为黑曲霉、乳酸链球菌、马克斯克鲁维酵母和东方伊萨酵母。

关键词: 自然发酵, 苹果渣, 微生物区系, 多相分类法

Abstract:

In order to investigate the microflora in different temperature layers of naturally fermented apple pomace, the
microorganisms were obtained by selective plate method and identified by morphology observation, physiological and biochemical
characteristics and molecular biological techniques (ITS region, 26S rRNA and 16S rRNA gene sequence analysis). The results
indicated that the major microorganisms of spontaneously fermented apple pomace included yeast and Lactobacillus, and
ten strains were isolated from four temperature layers. The dominant bacteria from four temperature layers were identified as
Aspergillus niger, Lactococcus lactis, Kluyveromyces marxianus and Issatchenkia orientalis, respectively.

Key words: natural fermentation, apple pomace, microflora, polyphasic taxonomy

中图分类号: