食品科学

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海参和海星皂苷对乳清酸诱导大鼠脂肪肝影响的比较研究

韩秀清,付雪媛,徐 杰,董 平,王静凤,薛长湖,王玉明*   

  1. 中国海洋大学食品科学与工程学院,山东 青岛 266003
  • 发布日期:2014-12-15

Effects of Sea Cucumber Saponins versus Starfish Saponins on Orotic Acid-Induced Fatty Liver in Rats

HAN Xiu-qing, FU Xue-yuan, XU Jie, DONG Ping, WANG Jing-feng, XUE Chang-hu, WANG Yu-ming*   

  1. College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China
  • Published:2014-12-15

摘要:

目的:研究并比较海参皂苷和海星皂苷对乳清酸诱导的长期大鼠非酒精性脂肪肝(nonalcoholic fatty liverdisease,NAFLD)的干预作用。方法:将雄性Wistar大鼠喂食1%乳清酸建立NAFLD模型,连续喂养6 周后,将大鼠分为乳清酸模型组(orotic acid group,OA)、海参皂苷组(sea cucumber saponins group,Scs)和海星皂苷组(starfish saponins group,Sfs),以未喂食OA的大鼠为正常对照组(control group,Con)继续喂养8 周后,分别测定大鼠肝脏甘油三酯(triglyceride,TG)、总胆固醇(total cholesterol,TC)、磷脂(phospholipid,PL)水平和血清TG、TC水平以及肝脏脂质合成、分解相关酶的酶活性和基因表达量。结果:海参皂苷和海星皂苷组肝脂及血脂水平均有明显下降,此外,二者均能抑制脂肪酸合成酶活性,下调脂肪酸合成基因表达水平,增强脂肪酸β-氧化相关酶活性。结论:海参皂苷和海星皂苷均能明显改善乳清酸长期诱导大鼠NAFLD,并具有类似的调控机制,其中海参皂苷和海星皂苷的改善效果相当。

关键词: 非酒精性脂肪肝, 海参皂苷, 海星皂苷, 乳清酸

Abstract:

Objective: In this research, the effects of sea cucumber saponins and starfish saponins on nonalcoholic fatty
liver induced by orotic acid (OA) in rats were comparatively investigated. Methods: Modified AIN-93G diet containing
1% orotic acid (OA diet) was used to establish NAFLD rat model. After 6-week feeding of OA diet, 24 male Wistar rats
were randomly divided into three groups (eight rats in each group) and then fed with the corresponding diets for 8 weeks:
OA group; OA diet; Scs group: OA diet containing 0.04% sea cucumber saponins; Sfs group: OA diet containing 0.04%
starfish saponins. The rats fed the modified AIN-93M diet were used as a control group (Con). After 14 weeks of feeding,
body weights, liver weights, visceral adipose weights, serum triglyceride (TG), serum total cholesterol (TC), liver functional
indexes, hepatic TG, hepatic TC and hepatic phospholipids (PL) were measured. The enzyme activities and gene expressions
involved in hepatic lipid metabolism were also determined. Results: Compared with the OA group, sea cucumber saponins
and starfish saponins significantly reduced hepatic TG and TC levels (P < 0.05). These two saponins alleviated the fatty liver
through inhibiting the enzymes activities and gene expressions involved in hepatic lipogenesis and enhancing the activities
of peroxisomal β-oxidation enzymes in liver. Conclusion: In summary, sea cucumber saponins and starfish saponins could
efficiently alleviate NAFLD induced by orotic acid in rats with similar regulatory mechanisms. Meanwhile, sea cucumber
saponins revealed similar lipid-lowering effects as sea starfish saponins.

Key words: nonalcoholic fatty liver disease, sea cucumber saponins, starfish saponins, orotic acid

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