食品科学

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表面等离子共振传感检测Cry2A蛋白

蔡 淼1,黄 新2,岳喜庆1,*   

  1. 1.沈阳农业大学食品学院,辽宁 沈阳 110866;2.中国检验检疫科学研究院植物检疫研究所,北京 100029
  • 出版日期:2014-12-25 发布日期:2014-12-29
  • 通讯作者: 岳喜庆
  • 基金资助:

    质检公益性行业科研专项(201410014)

Using a Surface Plasmon Resonance Sensor for the Detection of Bacillus thuringiensis Cry2A Protein

CAI Miao1, HUANG Xin2, YUE Xi-qing1,*   

  1. 1. College of Food Science, Shenyang Agricultural University, Shenyang 110866, China;
    2. Institute of Plant Quarantine, Chinese Academy of Inspection and Quarantine, Beijing 100029, China
  • Online:2014-12-25 Published:2014-12-29
  • Contact: YUE Xi-qing

摘要:

目的:建立利用表面等离子共振(surface plasmon resonance,SPR)传感器检测转基因植物中苏云金芽孢杆菌Cry2A蛋白的方法。方法:利用SPR检测技术,根据生物分子间的相互作用,在金片表面修饰Cry2A蛋白的特异性单克隆抗体,对不同质量浓度梯度的Cry2A蛋白进行检测研究。结果:该方法可有效地检测到Cry2A蛋白,检测灵敏度可达10 ng/mL,与同为抗虫基因编码的Cry1Ac和Cry2Ab蛋白未出现交叉反应。结论:利用SPR方法检测Cry2A蛋白操作简单,省时且灵敏度高、特异性强,可用于对Cry2A蛋白的定性检测。

关键词: 表面等离子共振传感器, Cry2A蛋白, 苏云金芽孢杆菌, 转基因植物

Abstract:

Purpose: To establish a method to detect Bacillus thuringiensis (Bt) Cry2A protein by surface plasmon resonance
(SPR) sensor. Methods: By using SPR based on interaction between biomolecules, the monoclonal antibodies specific to
the Cry2A protein were modified on the gold surface for the detection of this protein. Results: The detection limit of Cry2A
protein was 10 ng/mL by this method without cross-reaction with Cry1Ac or Cry2Ab protein. Conclusion: The SPR method
was simple, time-saving, sensitive, specific and applicable for the qualitative detection of Bt Cry2A protein.

Key words: surface plasmon resonance sensor, Cry2A protein, Bacillus thuringiensis, genetically modified plant

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