Cry2A蛋白的石英晶体微天平检测

doi:10.7506/spkx1002-6630-201424040

食品科学

Cry2A蛋白的石英晶体微天平检测

杜方1，黄新2，纪淑娟1,*

1.沈阳农业大学食品学院，辽宁沈阳 110866；
2.中国检验检疫科学研究院植物检疫研究所，北京 100029

出版日期:2014-12-25 发布日期:2014-12-29
通讯作者: 纪淑娟
基金资助:
质检公益性行业科研专项（201410014）

Detection of Cry2A Protein Based on Quartz Crystal Microbalance Technique

DU Fang1, HUANG Xin2, JI Shu-juan1,*

1. College of Food Science, Shenyang Agricultural University, Shenyang 110866, China;
2. Institute of Plant Quarantine, Chinese Academy of Inspection and Quarantine, Beijing 100029, China

Online:2014-12-25 Published:2014-12-29
Contact: JI Shu-juan

摘要/Abstract

摘要：

目的：建立检测苏云金芽孢杆菌Cry2A蛋白的石英晶体微天平（quartz crystal microbalance，QCM）传感方法。方法：根据抗原抗体相互作用原理，利用QCM技术，在金片表面修饰抗原所对应的单克隆抗体，对苏云金芽孢杆菌Cry2A蛋白进行检测研究。结果：该方法灵敏度达到1 μg/mL，特异性好、重复性高。结论：该方法有利于为苏云金芽孢杆菌蛋白检测提供新思路，可以应用到实际样品的检测，在农产品转基因检测和进出口检验检疫中具有很好的应用前景。

关键词: 石英晶体微天平, Cry2A蛋白, 转基因作物

Abstract:

Purpose: To establish a detection method for Cry2A protein by quartz crystal microbalance (QCM) sensing
method. Methods: By using QCM, based on the principle of the interaction between antigen and antibody, the specific
monoclonal antibody was modified on the gold surface for Cry2A protein detection. Results: The proposed method had a
high sensitivity of 1 μg/mL, good specificity, and sufficient repeatability. Conclusion: This method may provide a new idea
for the detection of Bacillus thuringiensis protein from genetically modified crops and thus has promising prospects for
application in import and export inspection and quarantine supervision work.

Key words: quartz crystal microbalance (QCM), Cry2A protein, genetically modified crop (GMC)

中图分类号:

杜方1，黄新2，纪淑娟1,*. Cry2A蛋白的石英晶体微天平检测[J]. 食品科学, doi: 10.7506/spkx1002-6630-201424040.

DU Fang1, HUANG Xin2, JI Shu-juan1,*. Detection of Cry2A Protein Based on Quartz Crystal Microbalance Technique[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201424040.

[1]	李葱葱，闫伟，夏蔚，董立明，龙丽坤，李飞武. 应用简并PCR方法检测转cry1A基因作物[J]. 食品科学, 2018, 39(14): 317-322.
[2]	王晨光，许文涛，朱鹏宇，付伟. 转基因组学分析技术研究进展[J]. 食品科学, 2015, 36(17): 288-295.
[3]	王森，李健爽，杜晓燕. 代谢组学在转基因作物非预期效应评价中的应用[J]. 食品科学, 2014, 35(9): 312-316.
[4]	蔡淼1，黄新2，岳喜庆1,*. 表面等离子共振传感检测Cry2A蛋白[J]. 食品科学, 2014, 35(24): 201-204.
[5]	许文涛，杨蓉，陆姣，张南，罗云波，何景，黄昆仑,. 转基因玉米59122品系的特异性检测[J]. 食品科学, 2011, 32(4): 139-142.

Cry2A蛋白的石英晶体微天平检测

Detection of Cry2A Protein Based on Quartz Crystal Microbalance Technique

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 5

编辑推荐

Metrics

本文评价