食品科学

• 基础研究 • 上一篇    下一篇

方格星虫多糖的分离纯化及单糖组成

董兰芳,张 琴*,许明珠   

  1. 广西壮族自治区海洋研究所,广西海洋生物技术重点实验室,广西 北海 536000
  • 出版日期:2015-01-15 发布日期:2015-01-16

Isolation, Purification and Monosaccharide Analysis of Polysaccharide from Sipunculus nudus

DONG Lanfang, ZHANG Qin*, XU Mingzhu   

  1. Key Laboratory of Marine Biotechnology of Guangxi, Guangxi Institute of Oceanology, Beihai 536000, China
  • Online:2015-01-15 Published:2015-01-16

摘要:

方格星虫多糖经碱法提取、三氯乙酸脱蛋白、DEAE-52纤维素柱层析、Sephadex G-100凝胶柱层析纯化得到精制方格星虫多糖(Sipunculus nudus polysaccharide,SNPS)。测定SNPS的理化性质和单糖组成,结果表明:SNPS为白色粉末状单一组分;经理化性质测定和紫外光谱分析,表明SNPS的多糖质量分数为99.35%,糖醛酸质量分数为22.21%,不含蛋白质和核酸,不含硫酸根;气相色谱分析结果表明方格星虫多糖SNPS主要由阿拉伯糖、葡萄糖和半乳糖组成,其物质的量比为1.16∶9.54∶1。本研究表明SNPS是从方格星虫中得到的均一组分纯多糖。

关键词: 方格星虫, 多糖, 纤维素, 凝胶, 单糖

Abstract:

Crude polysaccharide was obtained from Sipunculus nudus by alkali extraction and deproteinized by Sevag
method. The polysaccharide was sequentially purified by ion exchange chromatography on DEAE-52 cellulose column
and Sephadex G-100 Gel column. Then, the purified polysaccharide from Sipunculus nudus (SNPS) was characterized
for physicochemical properties and monosaccharide composition. The results showed that SNPS with a purity of 99.35%
contained 22.21% alduronic acid without protein, nuclein or sulfate ions. Gas chromatography analysis showed that the
monosaccharide compositions of SNPS were arabinose, glucose and galactose, with a molar ratio of 1.16:9.54:1. This study
demonstrated that SNPS was a homogeneous component obtained from Sipunculus nudus.

Key words: Sipunculus nudus, polysaccharide, cellulose, gel, monosacharide

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