食品科学

• 基础研究 • 上一篇    下一篇

雅安藏茶对脂肪酶的抑制作用

边金霖,郭金龙,李品武,杜 晓   

  1. 1.四川农业大学 国家茶检中心(四川)研发中心,四川 雅安 625014;
    2.国家茶叶质量检验中心(四川筹),四川 雅安 625000
  • 出版日期:2015-02-15 发布日期:2015-02-10

Inhibition of Lipase Activity by Ya’an Tibetan Tea

BIAN Jinlin, GUO Jinlong, LI Pinwu, DU Xiao   

  1. 1. Research and Development Center, National Tea Inspection Center (Sichuan), Sichuan Agricultural University, Ya’an 625014, China; 2. National Tea Quality Inspection Center (Sichuan), Ya’an 625000, China
  • Online:2015-02-15 Published:2015-02-10

摘要:

从雅安藏茶中系统萃取分离出7 个级分,使用酶标仪与96 孔板酶反应体系,对雅安藏茶水浸出物及各个级分抑制脂肪酶的活性成分进行系统筛选及评价。结果表明:雅安藏茶水浸出物(0.018~0.360 mg/mL)及其7 个级分的添加质量浓度(0.011~0.216 mg/mL)与其对脂肪酶活性的抑制作用之间均具有显著的量效关系;对脂肪酶活性的最大抑制率进行比较,雅安藏茶水浸出物为37.14%、儿茶素(catechin,C)级分为44.67%、茶黄素(theaflavin,TF)级分为39.46% 、茶褐素(theabromine,TB)级分为30.31%、茶红素Ⅱ(thearubiginsⅡ,TRsⅡ)级分为29.53%;结合主要成分含量和回归分析,各级分所含活性成分抑制脂肪酶活性能力大小顺序为:儿茶素>茶黄素>茶红素。

关键词: 雅安藏茶, 活性级分, 高通量筛选, 脂肪酶活性, 抑制作用

Abstract:

Seven fractions from the crude ethanol extract of Ya’an Tibetan tea were separated by organic solvents. The
seven fractions and the water extract of Ya’an Tibetan tea were analyzed by ELISA using a 96-well microplate reader for
lipase-inhibitory activities. The results showed that all the ethanol extract fractions (0.011–0.216 mg/mL) and the water
extract (0.018–0.360 mg/mL) possessed lipase-inhibitory activities with a significant concentration-effect relationship.
The maximum inhibitory rate of the water extract against lipase was 37.14%, while those of catechin (C), theaflavin (TF),
theabromine (TB) and thearubiginsⅡ (TRsⅡ) fractions extracted from Ya’an Tibetan tea were 44.67%, 39.46%, 30.31% and
29.53%, respectively. Lipase was inhibited in decreasing order by catechin, theaflavin and thearubigins from Ya’an Tibetan
tea, as indicated by analysis of the major components and regression analysis.

Key words: Ya’an Tibetan tea, active fraction, high-throughput screening, lipase activity, inhibition

中图分类号: