牛乳源金黄色葡萄球菌耐药性变迁及β-内酰胺类药物耐药基因分析

doi:10.7506/spkx1002-6630-201503028

食品科学

牛乳源金黄色葡萄球菌耐药性变迁及β-内酰胺类药物耐药基因分析

凡琴，刘书亮，吴聪明，韩新锋，周康，刘冬香，侯小刚

1.四川农业大学食品学院，四川雅安 625014；2.宜宾市南溪区食品药品监督管理局，四川宜宾 644100；
3.中国农业大学动物医学院，北京 100194

出版日期:2015-02-15 发布日期:2015-02-10

Drug Resistance Changes and Analysis of Beta-Lactam Drug Resistance Genes in Staphylococcus aureus from Fresh Milk

FAN Qin, LIU Shuliang, WU Congming, HAN Xinfeng, ZHOU Kang, LIU Dongxiang, HOU Xiaogang

1. College of Food Science, Sichuan Agricultural University, Ya’an 625014, China;
2. Nan Xi Food and Drug Administration, Yibin 644100, China;
3. College of Veterinary Medicine, China Agricultural University, Beijing 100194, China

Online:2015-02-15 Published:2015-02-10

摘要/Abstract

摘要：

采用肉汤微量稀释法，对203 株源于2006—2011年四川牛乳源金黄色葡萄球菌（Staphylococcus aureus，Sa）进行10 种常用抗生素药敏性检测，多重聚合酶链式反应（multiplex polymerase chain reaction，M-PCR）法对其耐甲氧西林基因（mecA）和β-内酰胺类药物耐药基因（blaZ）进行分析，了解Sa耐药性变迁。结果表明：牛乳源Sa菌株对青霉素、氨苄西林耐药率一直居高不下；对阿莫西林/克拉维酸、红霉素、甲氧苄啶/磺胺甲噁唑耐药率有增强趋势；对林可霉素、四环素耐药率呈降低趋势；对环丙沙星耐药率不定；对苯唑西林和头孢噻呋敏感；Sa分离株多重耐药率介于72.1%～100.0%，显示出不尽相同的耐药谱，却呈现出不断增宽的趋势。M-PCR检测显示，2006—2011年分离Sa菌株未从基因水平扩增出mecA基因；不同年度分离株均不同程度携带blaZ基因，不同年度分离株blaZ＋检出率介于49.1%～87.5%。药敏实验结果与相关耐药基因检测结果存在对应关系，部分菌株的耐药性与耐药基因的检出率不一致，推测还存在其他耐药机制。结论：四川地区牛乳源金黄色葡萄球菌耐药性不容乐观。

关键词: 生牛乳, 金黄色葡萄球菌, 耐药性, 多重聚合酶链式反应, &beta, -内酰胺类耐药基因

Abstract:

This study aimed to understand the changes in drug resistance of Staphylococcus aureus (Sa). A total of 203
strains of Sa collected during 2006 to 2011 were tested against 10 antibiotics by broth micro-dilution method for determining
their antibiotic susceptibility. With multiplex polymerase chain reaction (M-PCR) method, the methicillin-resistant gene
(mecA) and beta-lactam drug resistance gene (blaZ) of Sa were analyzed. The results showed that the resistance rates of
Sa isolates to penicillin and ampicillin acid were high. An increasing trend was observed for the resistance rates of Sa
to amoxicillin/clavulanic, erythromycin, and trimethoprim/sulfamethoxazole. There was an uncertain resistance rate to
ciprofloxacin whereas the resistance rates of Sa isolates to clindamycin and tetracycline showed a decreasing trend. All the
strains tested were sensitive to both oxacillin and ceftiofur. The multidrug-drug resistant rates of Sa from different years
ranged from 72.1% to 100.0%, with different drug-resistant spectra, but at the same time, they presented a continuously widened
trend. M-PCR analysis showed that no mecA gene was amplified from Sa strains collected during 2006 to 2011. During different
years, more or less isolates carrying the gene blaZ were detected, and the detection rates of blaZ+ from different years ranged
from 49.1% to 87.5%. There was a certain relationship between the susceptibility test results and the related drug resistance gene
detection results. The detection rates of some drug-resistant strains were inconsistent with their resistance genes suggesting that
there are other resistance mechanisms for Sa. The drug resistance of Sa from fresh milk in Sichuan was not optimistic.

Key words: fresh milk, Staphylococcus aureus, drug resistance, multiplex polymerase chain reaction (M-PCR), β-lactam drug resistance genes

中图分类号:

R155.31

凡琴，刘书亮，吴聪明，韩新锋，周康，刘冬香，侯小刚. 牛乳源金黄色葡萄球菌耐药性变迁及β-内酰胺类药物耐药基因分析[J]. 食品科学, doi: 10.7506/spkx1002-6630-201503028.

FAN Qin, LIU Shuliang, WU Congming, HAN Xinfeng, ZHOU Kang, LIU Dongxiang, HOU Xiaogang. Drug Resistance Changes and Analysis of Beta-Lactam Drug Resistance Genes in Staphylococcus aureus from Fresh Milk[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201503028.

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牛乳源金黄色葡萄球菌耐药性变迁及β-内酰胺类药物耐药基因分析

Drug Resistance Changes and Analysis of Beta-Lactam Drug Resistance Genes in Staphylococcus aureus from Fresh Milk

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