食品科学

• 营养卫生 • 上一篇    下一篇

食用香甜味沙拉酱对β-胡萝卜素吸收的影响

李超超,增田泰伸,木村守,臼田美香,王 唯,徐文斌,奚印慈   

  1. 1.上海海洋大学食品学院,上海 201306;2.日本丘比株式会社,东京 1820002
  • 出版日期:2015-02-15 发布日期:2015-02-10

Effect of Sweet Mayonnaise Intake on β-Carotene Absorption in Rats

LI Chaochao, YASUNOBU Masuda, MIKA Usuda, MAMORU Kimura, WANG Wei, XU Wenbin, XI Yinci   

  1. 1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China;
    2. Japan Kewpie Corporation, Tokyo 1820002, Japan
  • Online:2015-02-15 Published:2015-02-10

摘要:

目的:通过动物实验、体外实验确认食用香甜味沙拉酱对β-胡萝卜素吸收的影响效果。方法:采用高效液相色谱(high performance liquid chromatography,HPLC)技术进行β-胡萝卜素定量分析;参考93号饲料(AIN-93)配方,分组连续喂养大鼠12 d,测定不同组别大鼠肝脏中β-胡萝卜素的含量;配制人工胃液,分别将磨碎的芒果与香甜味沙拉酱和大豆油混合,模拟胃液蠕动搅拌不同时间,并测定油相中β-胡萝卜素含量;分别给大鼠灌胃等量的β-胡萝卜素以及油量相等的香甜味沙拉酱与大豆油,比较香甜味沙拉酱与大豆油在大鼠胃中停留的时间并测定油相中β-胡萝卜素含量。结果:大鼠肝脏中β-胡萝卜素含量为香甜味沙拉酱组>大豆油组(P<0.05);体外模拟实验发现,芒果更容易分散于香甜味沙拉酱体系,油相中β-胡萝卜素含量为香甜味沙拉酱组>大豆油组,搅拌60 min组>搅拌30 min组(P<0.05);大鼠在灌胃30 min后胃液中β-胡萝卜素含量为香甜味沙拉酱组>大豆油组(P<0.05),且香甜味沙拉酱在胃中停留的时间更长。结论:食用香甜味沙拉酱比单纯食用大豆油更能够促进β-胡萝卜素的吸收转化;香甜味沙拉酱更能促进富含β-胡萝卜素水果中的β-胡萝卜素的吸收。

关键词: 香甜味沙拉酱, &beta, -胡萝卜素, 大豆油, 高效液相色谱, 肝脏, 胃液

Abstract:

Purpose: To investigate the effect of sweet mayonnaise intake on β-carotene absorption by both animal and in
vitro experiments. Methods: The concentration of β-carotene was measured by high performance liquid chromatography
(HPLC). Rats were continuously fed an AIN-93 based diet for 12 days and β-carotene concentrations in rat livers from
different groups were measured at the end of the administration period. Sweet mayonnaise or soybean oil was stirred
together with grated mango in artificial gastric juice under simulated gastric peristalsis forces and β-carotene concentration
in the oil phase was measured at different time points. Equal amounts of β-carotene and soybean oil were orally administered
to each rat, and then the residence times of sweet mayonnaise and soybean oil in the rat stomach were observed and β-carotene
concentration in the oil phase was measured. Results: β-carotene concentration in the liver of sweet mayonnaise-fed rats
was significantly higher than in that of soybean oil-fed rats (P < 0.05).The in vitro experiments showed that the mango was
more easily dispersed in the sweet mayonnaise. β-carotene concentration in the oil phase of sweet mayonnaise-fortified diet
was significantly higher than in that of soybean oil-fortified diet, and an increase was achieved by extending the stirring
time from 30 to 60 min (P < 0.05). At 30 min after administration, the gastric juice of sweet mayonnaise-fed rats contained higher
β-carotene concentration than that of soybean oil-fed rats (P < 0.05), and the residence time of sweet mayonnaise in the rat stomach
was longer than that of soybean oil. Conclusions: Sweet mayonnaise intake more easily promotes β-carotene absorption than single
soybean oil intake; sweet mayonnaise can enhance the absorption of β-carotene from β-carotene-rich fruits.

Key words: sweet mayonnaise, β-carotene, soybean oil, high performance liquid chromatography, liver, gastric juice

中图分类号: