食品科学

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吸附树脂对蛹虫草黄酮纯化工艺条件优化

陈晓侠1,宋 渊2,张纪柏3,王增利1,*   

  1. 1.中国农业大学食品科学与营养工程学院,北京 100083;2.中国农业大学生物学院,北京 100193;
    3.国内贸易工程设计研究院,北京 100069
  • 出版日期:2015-02-25 发布日期:2015-02-16
  • 通讯作者: 王增利

Purification of Flavonoids from Cordyceps militaris by Macroporous Adsorption Resin

CHEN Xiaoxia1, SONG Yuan2, ZHANG Jibai3, WANG Zengli1,*   

  1. 1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China;
    2. College of Biological Sciences, China Agricultural University, Beijing 100193, China;
    3. Internal Trade Engineering Design and Research Institute, Beijing 100069, China
  • Online:2015-02-25 Published:2015-02-16
  • Contact: WANG Zengli

摘要:

以蛹虫草黄酮粗提物为研究对象,分析黄酮纯化过程中树脂种类、上样体积、淋洗液pH值、洗脱液体积分数与体积及树脂重复使用次数多种影响因素,优化吸附树脂对黄酮的分离纯化工艺。通过对AB-8、D-101、NKA-9和NKA-Ⅱ 4 种吸附树脂对蛹虫草黄酮的静态吸附、静态解吸和静态吸附动力学等特性的研究,发现AB-8吸附树脂对蛹虫草黄酮有较高的吸附速率和单位吸附量,且易于解吸,是蛹虫草黄酮分离的理想树脂。通过优化实验,确定AB-8吸附树脂对蛹虫草黄酮分离纯化的最优工艺条件为树脂装柱体积100 mL时,上样体积40.0 mL、黄酮上样量47.536 mg、淋洗和洗脱速率2 BV/h、淋洗液pH 5、洗脱液乙醇体积分数和洗脱体积分别为85%和500 mL,树脂重复使用次数为2 次,在此条件下,蛹虫草黄酮的回收率在65%以上,纯度在17%以上,具有良好的分离纯化效果。

关键词: 蛹虫草, 黄酮, 吸附树脂, 纯化

Abstract:

The purification of flavonoids from Cordyceps militaris using macroporous resin was optimized by investigating
several process parameters including resin type, sample volume, eluent pH, concentration and volume, and repeated use
of the selected resin. Static adsorption and desorption, and kinetic studies of static adsorption demonstrated that AB-8 was
selected as the better adsorbent with higher adsorption rate and adsorption capacity per unit mass for Cordyceps militaris
flavonoids compared with D-101, NKA-9 and NKA-Ⅱ. As determined by optimization experiments, the recovery of
flavonoids was more than 65% with a purity of over 17% when 40.0 mL of the sample (47.536 mg of flavonoids) was loaded
onto a 100-mL column at a flow rate of 2 BV/h of AB-8 followed by elution with 500 mL of 85% ethanol acidified to pH 5
also at flow rate of 2 BV/h and the resin was used to repeat the process once more.

Key words: Cordyceps militaris, flavonoids, macroporous resins, purification

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