食品科学

• 基础研究 • 上一篇    下一篇

干燥方式对芡实功能性成分含量及抗氧化活性的影响

王 红1,吴启南1,2,*,蒋 征1,樊修和1,谷 巍1,乐 巍1   

  1. 1.南京中医药大学药学院,江苏 南京 210023;2.江苏省中药资源产业化过程协同创新中心,江苏 南京 210023
  • 出版日期:2015-04-15 发布日期:2015-05-05

Effects of Different Drying Methods on Functional Component Contents and Antioxidant Activity of Euryale Semen

WANG Hong1, WU Qinan1,2,*, JIANG Zheng1, FAN Xiuhe1, GU Wei1, YUE Wei1   

  1. 1. College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China;
    2. Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing 210023, China
  • Online:2015-04-15 Published:2015-05-05

摘要:

目的:以芡实中总多糖、总酚、总黄酮、不同构型VE含量及抗氧化活性为指标,考察芡实适宜的干燥方式。方法:以紫外分光光度法测定芡实中的总多糖、总酚、总黄酮含量及抗氧化活性,利用高效液相色谱法测定芡实中不同构型VE含量为质量指标,综合评价6 种不同干燥方法(阴干、晒干、烘干、真空冷冻干燥、红外干燥、微波干燥)对芡实品质的影响。结果:采用微波干燥能够最大程度地保留样品中总多糖、总酚和总黄酮类物质;在真空冷冻干燥处理条件下,芡实中不同构型VE含量最高且清除1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基、2,2’-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐自由基(2,2’-azinobis-(3-ethylbenzthiazoline-6-sulphonate),ABTS+·)的能力最强;相关性分析显示,各不同构型VE含量与抗氧化活性指标具有显著相关性。结论:真空冷冻干燥在最大程度积累VE各类构型成分的基础上,其产品表现出最佳的抗氧化活性,并在一定程度上尽可能地降低了芡实总多糖、总酚及总黄酮类成分的损失,可作为芡实产地加工适宜的干燥方法。

关键词: 干燥方法, 芡实, 功能性成分, 抗氧化活性

Abstract:

Objective: The contents of total polysaccharides, total phenols, total flavonoids and different configurations
of vitamin E and antioxidant activity were considered as indicators to select a suitable drying method for Euryale
Semen. Methods: Ultraviolet (UV) spectroscopy was applied to determine antioxidant activity and the contents of total
polysaccharides, total phenols and total flavonoids and different configuration of vitamin E were quantified by high
performance liquid chromatography (HPLC). The effects of six different drying methods including shade drying, sun
drying, cabinet drying, vacuum-freeze drying, infrared drying and microwave drying on the quality of Euryale Semen
were evaluated. Results: Microwave drying could largely retain total polysaccharides, total phenols and total flavonoids in
samples. But the highest contents of different configuration of vitamin E were accumulated and the ability to scavenge DPPH
free radicals and ABTS+· was the strongest in vacuum freeze-dried samples. Meanwhile, a significant correlation existed
between vitamin E and antioxidant activity. Conclusion: Vacuum freeze drying can be regarded as a suitable method for
processing Euryale Semen because it not only can largely accumulate the content of vitamin E to result in the best antioxidant
activity, but also can reduce the loss of total polysaccharides, total phenols and total flavonoids to the largest extent.

Key words: drying method, Euryale Semen, functional component, antioxidant activity

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