果蔬生防菌葡萄有孢汉逊酵母CM2糖蜜培养基的优化

doi:10.7506/spkx1002-6630-201507022

食品科学

果蔬生防菌葡萄有孢汉逊酵母CM2糖蜜培养基的优化

蔡子康，王晓霞，秦晓杰，杨蓉，司琳媛，肖红梅*

南京农业大学食品科技学院，江苏南京 210095

出版日期:2015-04-15 发布日期:2015-05-05

Optimization of Molasses Medium Composition for Hanseniaspora uvarum CM2 as a Biocontrol Yeast

CAI Zikang, WANG Xiaoxia, QIN Xiaojie,YANG Rong, SI Linyuan, XIAO Hongmei*

College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China

Online:2015-04-15 Published:2015-05-05

摘要/Abstract

摘要：

以葡萄有孢汉逊酵母CM2为研究对象，细胞生物量为测定指标，利用甘蔗糖蜜作为发酵原料，对糖蜜培养基的优化进行较为全面的探讨。采用煮沸法、磷酸法、硫酸法、活性炭法、亚铁氰化钾法对糖蜜进行预处理，结果表明硫酸处理法优于其他处理方法。通过单因素试验研究糖蜜质量浓度、氮源及其添加量、初始pH值、磷酸盐、硫酸镁、氯化钠等培养基组分对CM2生长的影响；采用Plackett-Burman试验设计、最陡爬坡试验和中心组合试验设计对培养基组分进行优化，得到优化培养基组分：预处理后糖蜜78.9 g/L、酵母粉2.2 g/L、初始pH 6.0。通过验证实验获知与PDB培养基相比，优化后糖蜜培养基使CM2生物量提高了83.1%。

关键词: 葡萄有孢汉逊酵母, 甘蔗糖蜜, Plackett-Burman试验设计, 培养基, 生物量

Abstract:

The composition of a sugarcane molasses-based medium used to culture Hanseniaspora uvarum CM2 was
optimized for enhanced biomass production. The molasses was pretreated by boiling or adding phosphoric acid, sulfuric
acid, potassium ferrocyanide and activated carbon, respectively. Comparing their impacts on the growth of the antagonistic
yeast CM2, sulfuric acid addition was considered the method of choice. Molasses concentration, the type and concentration
of nitrogen source, initial pH, phosphate, magnesium sulfate, and sodium chloride were studied for their influence on the
biomass production of CM2 by single factor design. Plackett-Burman design and response surface methodology were used
to optimize the medium components as pretreated molasses 78.9 g/L, yeast powder 2.2 g/L and initial pH 6.0. The optimal
medium could result in an 83.1% enhancement in the biomass production of CM2.

Key words: Hanseniaspora uvarum, sugarcane molasses, Plackett-Burman experimental design, medium, biomass

中图分类号:

TS201.1

蔡子康，王晓霞，秦晓杰，杨蓉，司琳媛，肖红梅*. 果蔬生防菌葡萄有孢汉逊酵母CM2糖蜜培养基的优化[J]. 食品科学, doi: 10.7506/spkx1002-6630-201507022.

CAI Zikang, WANG Xiaoxia, QIN Xiaojie,YANG Rong, SI Linyuan, XIAO Hongmei*. Optimization of Molasses Medium Composition for Hanseniaspora uvarum CM2 as a Biocontrol Yeast[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201507022.

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果蔬生防菌葡萄有孢汉逊酵母CM2糖蜜培养基的优化

Optimization of Molasses Medium Composition for Hanseniaspora uvarum CM2 as a Biocontrol Yeast

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