食品科学

• 营养卫生 • 上一篇    下一篇

霍山石斛中不同多糖组分的保肝活性

田长城1,2,罗建平1,*   

  1. 1.合肥工业大学生物与食品工程学院,安徽 合肥 230009;2.蚌埠学院生物与食品工程系,安徽 蚌埠 233030
  • 出版日期:2015-04-15 发布日期:2015-05-05

Hepatoprotective Effects of Different Polysaccharides from Dendrobium huoshanense

TIAN Changcheng1,2, LUO Jianping1,*   

  1. 1. School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China;
    2. Department of Biology and Food Engineering, Bengbu University, Bengbu 233030, China
  • Online:2015-04-15 Published:2015-05-05

摘要:

采用热水浸提和DEAE-纤维素阴离子交换法从霍山石斛中分离出4 种多糖组分(DHP1、DHP2、DHP3和DHP4),比较了4 种多糖组分对四氯化碳(CCl4)肝毒性的保护作用。结果表明:口服DHP1显著降低了CCl4诱导的小鼠血清谷丙转氨酶(alanine aminotransferase,ALT)和谷草转氨酶(aspartate aminotransferase,AST)活性的升高,削弱了肝组织中丙二醛(malondialdehyde,MDA)的表达,同时恢复了肝组织中超氧化物歧化酶(superoxide dismutase,SOD)和过氧化氢酶(catalase,CAT)的活性。因此,DHP1可能是霍山石斛中一种潜在的保肝多糖成分。

关键词: 霍山石斛, 多糖, 四氯化碳, 肝损伤

Abstract:

Four polysaccharides (DHP1, DHP2, DHP3 and DHP4) were isolated and purified from young seedlings of
Dendrobium huoshanense by hot water extraction and DEAE cellulose anion exchange chromatography. The objective of
this study was to investigate the protective effects of the four polysaccharides on carbon tetrachloride (CCl4)-induced acute
liver injury in mice. The results showed that oral administration of DHP1 significantly decreased the levels of serum alanine
aminotransferase (ALT) and aspartate aminotransferase (AST), reduced the content of hepatic malondialdehyde (MDA) and
restored the activities of superoxide dismutase (SOD) and catalase (CAT) in the CCl4-treated mice, suggesting that DHP1 is
a potential hepatoprotective compound in D. huoshanense.

Key words: Dendrobium huoshanense, polysaccharide, carbon tetrachloride, liver injury

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