沉淀聚合法制备葛根素印迹微球及其固相萃取葛根粉

doi:10.7506/spkx1002-6630-201510003

食品科学

沉淀聚合法制备葛根素印迹微球及其固相萃取葛根粉

许苗苗1，王素素1，李辉1,2,*，黄宇琳1，逯翠梅1，蒋剑波1

1.吉首大学化学化工学院，湖南吉首 416000；
2.吉首大学植物资源保护与利用湖南省高校重点实验室，湖南吉首 416000

出版日期:2015-05-25 发布日期:2015-05-08
通讯作者: 李辉
基金资助:
国家自然科学基金面上项目（21077042）；湖南省科技厅计划项目（2012SK3145）

Preparation of Puerarin-Imprinted Polymer Microspheres by Precipitation Polymerization Technique for Use in Solid Phase Extraction of Flavonoids from Pueraria Root Powder

XU Miaomiao1, WANG Susu1, LI Hui1,2,*, HUANG Yulin1, LU Cuimei1, JIANG Jianbo1

1. College of Chemistry and Chemical Engineering, Jishou University, Jishou 416000, China;
2. Key Laboratory of Plant Resource Conservation and Utilization, Jishou University, Jishou 416000, China

Online:2015-05-25 Published:2015-05-08
Contact: LI Hui

摘要/Abstract

摘要：

以葛根素为模板分子，甲基丙烯酸为功能单体，乙二醇二甲基丙烯酸酯为交联剂沉淀聚合法制备葛根素印迹聚合物微球。用扫描电镜观察微球形貌，静态吸附法测试聚合物的吸附行为。探讨分子印迹聚合物（molecularly imprinted polymers，MIPs）对葛根粗提液中葛根素的吸附、解吸附及固相萃取效能。结果表明，优化的葛根素MIP3对纯模板的静态吸附量达37.9 mg/g；对粗提液中目标化合物的吸附率为（96.3±1.81）%，以水、甲醇-醋酸（9∶1，V/V）及50%甲醇溶液为洗脱剂的单次解吸率为27%～34%，用水从MIP3上解吸出的总溶液经脱除溶剂后，所获粗品葛根素含量最高，达（37.4±2.87）%。在优化条件下，通过MIP3固相萃取葛根粗提液，葛根素回收率达71.6%，产品纯度高于75%。

关键词: 分子印迹聚合物, 葛根素, 沉淀聚合法, 吸附, 固相萃取

Abstract:

Puerarin-imprinted polymer microspheres (MIPM) were prepared by precipitation polymerization technique using
puerarin, methacrylic acid (MAA) and ethylene glycol dimethylacrylate (EGDMA) as the template, functional monomer and
cross-linker, respectively. Scanning electron microscope (SEM) was used for evaluating the morphology of polymer and a
static method was used for characterizing the adsorption behavior. The application of the MIPM for adsorption, desorption
and solid phase extraction of puerarin from crude pueraria root extract was explored. Results indicated that the adsorption
capacity for the optimized MIPM (MIP3) toward the pure template reached 37.9 mg/g, with an adsorption percentage of
(96.3 ± 1.81)% toward the target compound in the crude extract. The desorption percentage varied within the range of
27%–34% when water, methanol-acetic acid (9:1, V/V ) and 50% aqueous methanol were used as the eluents, respectively.
Furthermore, the product desorbed from the MIP3 using water as the eluent contained the highest content of puerarin
(37.4 ± 2.87)%. Puerarin product with 75% purity can be obtained by MIP3 using single-step solid phase extraction with a
puerarin recovery of 71.6%.

Key words: molecularly imprinted polymer, puerarin, precipitation polymerization, adsorption, solid phase extraction

中图分类号:

TS202.3

许苗苗1，王素素1，李辉1,2,*，黄宇琳1，逯翠梅1，蒋剑波1. 沉淀聚合法制备葛根素印迹微球及其固相萃取葛根粉[J]. 食品科学, doi: 10.7506/spkx1002-6630-201510003.

XU Miaomiao1, WANG Susu1, LI Hui1,2,*, HUANG Yulin1, LU Cuimei1, JIANG Jianbo1. Preparation of Puerarin-Imprinted Polymer Microspheres by Precipitation Polymerization Technique for Use in Solid Phase Extraction of Flavonoids from Pueraria Root Powder[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201510003.

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沉淀聚合法制备葛根素印迹微球及其固相萃取葛根粉

Preparation of Puerarin-Imprinted Polymer Microspheres by Precipitation Polymerization Technique for Use in Solid Phase Extraction of Flavonoids from Pueraria Root Powder

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