食品科学

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紫薯中绿原酸类化合物标准品的制备

李 甫1,陈 斌1,李联洪2,王明奎1   

  1. 1.中国科学院成都生物研究所,四川 成都 610041;2.成都曼思特生物科技有限公司,四川 成都 610000
  • 出版日期:2015-06-25 发布日期:2015-06-12
  • 基金资助:

    四川省科技支撑计划项目(2012SZ0054);“十二五”国家科技支撑计划项目(2011BAC09B04)

Preparation of High-Purity Chlorogenic Acid Derivatives from Purple Sweet Potato

LI Fu1, CHEN Bin1, LI Lianhong2, WANG Mingkui1   

  1. 1. Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China;
    2. Chengdu MUST Bio-technology Company Limited, Chengdu 610000, China
  • Online:2015-06-25 Published:2015-06-12

摘要:

从紫薯中提取制备高纯度、市场价值较高的绿原酸类化合物标准品。紫薯切片,采用沸水提取、大孔树脂吸附和凝胶柱色谱分段,然后以反相制备液相色谱获得高纯标准品,最后以核磁共振技术确定化学结构。从紫薯中制备得到异绿原酸A、异绿原酸B、异绿原酸C和3,4,5-O-三咖啡酰基奎宁酸,高效液相色谱检测表明这些化合物的纯度均在98%以上。本研究建立的制备工艺可高效地将紫薯中绿原酸类化合物富集,并快速获得高纯样品,有效地避免了紫薯花色苷类化合物对产品色泽和纯度的影响。

关键词: 紫薯, 绿原酸类化合物, 标准品, 分离, 制备

Abstract:

The purpose of the present study was to prepare high-purity and high-value chlorogenic acid derivatives from
purple sweet potato. Purple sweet potato slices were extracted with hot water. The extracted compounds were separated with
D72 macroporous resin, sephadex LH-20 column chromatography and preparative high performance liquid chromatography
(HPLC) to obtain high-purity chlorogenic acid analogues which were identified as isochlorogenic acid A, isochlorogenic acid
B, isochlorogenic acid C and 3,4,5-O-tricaffeoyl quinic acid, respectively, by nuclear magnetic resonance technique. HPLC
analysis suggested that the purities of these analogues were all over 98%. The developed method can be used to rapidly
obtain standard samples of chlorogenic acid derivatives from purple sweet potato, while avoiding effectively the negative
effects of the anthocyanins on product color and purity.

Key words: purple sweet potato, chlorogenic acid derivative, standard sample, isolation, preparation

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