霍山石斛不同提取物抗小鼠亚急性酒精性肝损伤活性的比较研究

doi:10.7506/spkx1002-6630-201513042

摘要/Abstract

摘要：

目的：比较霍山石斛不同提取物抗小鼠亚急性酒精性肝损伤的活性。方法：制备霍山石斛冷冻干燥物、水提物、水提醇溶物、水提醇沉物、水提粗多糖5 种提取物；以连续灌胃30%乙醇的小鼠为亚急性酒精性肝损伤模型，以霍山石斛不同提取物连续灌胃30 d后，称量小鼠体质量及肝质量，测定血清中谷丙转氨酶（alanineaminotransferase，ALT）、谷草转氨酶（aspartate aminotransferase，AST）、碱性磷酸酶（alkaline phosphatase，ALP）活性，以及总胆固醇（total cholesterol，TC）、甘油三酯（triglyceride，TG）、高密度脂蛋白胆固醇（highdensity lipoprotein cholesterol，HDL-C）、低密度脂蛋白胆固醇（low density lipoprotein cholesterol，LDL-C）含量，同时测定肝脏中乙醇脱氢酶（alcohol dehydrogenase，ADH）、乙醛脱氢酶（acetaldehyde dehydrogenase，ALDH）、谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-Px）、超氧化物歧化酶（superoxide dismutase，SOD）活性，丙二醛（malondialdehyde，MDA）、谷胱甘肽（glutathione，GSH）水平，并检查肝组织损伤病理变化。结果：霍山石斛水提醇溶物抗亚急性酒精性肝损伤的活性最差，醇沉物、水提物、冷冻干燥物具有一定的肝损伤保护活性，水提粗多糖各个剂量组均可显著改善肝脏组织损伤和脂肪变性（P＜0.05），降低血清ALT、AST、ALP活性和LDL-C、TC、TG水平，提高血清HDL-C含量，增强肝组织ADH、ALDH、SOD、GSH-Px活性，减少肝组织GSH损耗并抑制肝组织MDA含量增加。结论：多糖是霍山石斛抗小鼠亚急性酒精性肝损伤的功能因子。

关键词: 霍山石斛, 提取物, 亚急性酒精性肝损伤

Abstract:

Objective: To compare the protective effects of different extracts from Dendrobium huoshanense against alcoholinduced
subacute liver injury in mice. Methods: Freeze-dried Dendrobium huoshanense and its different extracts including
water extract, alcohol-soluble extract, alcohol-insoluble extract and crude polysaccharides were prepared. Mouse liver injury
was induced by oral administration of 30% alcohol and different extracts were employed to intervene in the liver injury.
After continuous treatment for 30 days, mouse body weight and liver weight were measured, liver pathological changes were
examined, the contents of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase
(ALP), total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein
cholesterol (LDL-C) were determined, and the levels of hepatic alcohol dehydrogenase (ADH), acetaldehyde dehydrogenase
(ALDH), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), malonaldehyde (MDA), and glutathione (GSH)
were determined. Results: The protective effects of freeze-dried Dendrobium huoshanense, and its water extract and alcoholinsoluble
extract against alcohol-induced subacute liver injury were better than that of alcohol-soluble extract, but lower
than that of crude polysaccharides. Liver tissue damage and steatosis in mice subjected to 30% alcohol were ameliorated by
oral administration with crude polysaccharides, the levels of ALT, AST, ALP, LDL-C, TC and TG in serum were reduced,
the level of HDL-C in serum and the activities of ADH, ALDH, SOD and GSH-Px in liver were enhanced, and the loss of
GSH and the increase of MDA in liver were suppressed. Conclusion: Polysaccharides are the major functional factors in
Dendrobium huoshanense protecting liver tissue from alcohol-induced injury.

Key words: Dendrobium huoshanense, extract fractions, subacute alcoholic liver injury

中图分类号:

Q946.3

孟海涛，汪鹤，查学强，潘利华，罗建平*. 霍山石斛不同提取物抗小鼠亚急性酒精性肝损伤活性的比较研究[J]. 食品科学, doi: 10.7506/spkx1002-6630-201513042.

MENG Haitao, WANG He, ZHA Xueqiang, PAN Lihua, LUO Jianping*. Comparison of Hepatoprotective Effects of Different Extracts from Dendrobium huoshanense against Alcohol-Induced Subacute Liver Injury in Mice[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201513042.

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霍山石斛不同提取物抗小鼠亚急性酒精性肝损伤活性的比较研究

Comparison of Hepatoprotective Effects of Different Extracts from Dendrobium huoshanense against Alcohol-Induced Subacute Liver Injury in Mice

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