食品科学

• 营养卫生 • 上一篇    下一篇

膳食5’-核苷酸对酒精性肝损伤大鼠肠道菌群的影响

蔡夏夏,鲍 雷,王 楠,徐美虹,李 迪,武 欣,张召锋,李 勇*   

  1. 北京大学公共卫生学院营养与食品卫生学系,北京 100191
  • 出版日期:2015-08-15 发布日期:2015-08-17

Effects of Dietary 5’-Nucleotides on Gut Microbiota in Rats with Ethanol-Induced Liver Injury

CAI Xiaxia, BAO Lei, WANG Nan, XU Meihong, LI Di, WU Xin, ZHANG Zhaofeng, LI Yong*   

  1. Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China
  • Online:2015-08-15 Published:2015-08-17

摘要:

目的:研究膳食5’-核苷酸对酒精性肝损伤大鼠肠道菌群的影响,并进一步探讨可能的作用机制。方法:雄性Wistar大鼠随机分为对照组、酒精组、葡萄糖等热量对照组、普通饲料组、质量分数0.04%、0.16% 5’-核苷酸干预组,连续饲养7 周,测定大鼠体质量、脏体比、血清乙醇体积分数、血清转氨酶活力、血脂水平及总蛋白含量等相关指标;平板培养大鼠粪便中的乳酸杆菌、双歧杆菌、大肠杆菌、肠球菌,计算大鼠粪便中各种细菌的菌落数。结果:膳食5’-核苷酸能够增加酒精引起的大鼠体质量降低,轻度抑制血清乙醇体积分数的升高,抑制酒精引起丙氨酸氨基转移酶、乳酸脱氢酶活力和甘油三酯水平等升高(P<0.05),增加血清总蛋白、白蛋白及球蛋白含量(P<0.05);并能够增加肝体比,减少盲体比(P<0.05);膳食5’-核苷酸能够增加肠道乳酸杆菌数量,同时减少大肠杆菌及肠球菌的数量(P<0.05)。结论:膳食5’-核苷酸能够改善酒精引起的大鼠肝脏损伤,调节肠道菌群可能是其作用机制之一。

关键词: 5&rsquo, -核苷酸, 酒精性肝损伤, 肠道菌群, 丙氨酸氨基转移酶, 酒精性脂肪性肝炎

Abstract:

Purpose: To observe the effects of dietary 5’-nucleotides on the gut microbiota of rats with alcoholic liver injury.
Methods: Male Wistar rats were given either alcohol or glucose by gavage for 7 weeks. A subgroup of rats was also fed
with diets including 0.04% or 0.16% 5’-nucleotides. Body weights, liver to body weight ratio, and the levels of serum
alcohol concentration, transaminase activities, triglycerides and total proteins were tested. Stool cultures were performed
at the 7th week, and the colony-forming units per gram stool were determined. Results: The alanine aminotransferase,
triglycerides, lactate dehydrogenase and total bilirubin were significantly elevated, and total proteins, albumin and
globulin were significantly decreased in alcoholic live injury group when compared to vehicle or glucose groups. Dietary
5’-nucleotides could increase body weights and the ratio of liver weight to body weight, and decrease the levels of serum
ethanol, alanine aminotransferase, triglycerides, lactate dehydrogenase and total bilirubin in rats with alcoholic live injury.
Total protein, albumin and globulin were significantly increased in nucleotide groups when compared to alcoholic live injury
group. Nucleotide groups had significantly increased number of benefical lactobacilli, and reduced number of pathogenic
Escherichia coli and enterococci. Conclusion: Dietary 5’-nucleotides could improve ethanol-induced liver injury, and the
mechanism may be associated with regulation of the gut microbiota.

Key words: 5’-nucleotides, alcoholic liver injury, gut microbiota, alanine aminotransferase, alcoholic steatohepatitis

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