食品科学

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渗透汽化原位分离耦合拜氏梭菌丁醇发酵的研究

刘晓洁1,2,沈兆兵1,2,刘 莉1,*,史吉平1,3,*   

  1. 1.中国科学院上海高等研究院,上海 201210;2.中国科学院大学,北京 100049;
    3.上海科技大学生命科学与技术学院,上海 201210
  • 出版日期:2015-09-15 发布日期:2015-09-11

Butanol Fermentation by Clostridium beijerinckii Integrated with in situ Pervaporation

LIU Xiaojie1,2, SHEN Zhaobing1,2, LIU Li1,*, SHI Jiping1,3,*   

  1. 1. Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China;
    2. University of Chinese Academy of Sciences, Beijing 100049, China;
    3. School of Life Science and Technology, Shanghai Tech University, Shanghai 201210, China
  • Online:2015-09-15 Published:2015-09-11

摘要:

以筛选得到的聚二甲基硅氧烷(polydimethylsiloxane,PDMS)-聚偏氟乙烯(polyvinylidene fluoride,PVDF)复合膜为分离用膜,开展了拜氏梭菌(Clostridium beijerinckii)ZL01丁醇发酵与渗透汽化原位分离耦合的研究,结果表明:分批发酵-渗透汽化原位分离耦合与分批发酵相比,初始葡萄糖质量浓度从50 g/L提高至90 g/L;在90 g/L的初始葡萄糖质量浓度下,发酵结束时发酵液和渗透液中的丁醇总产量从13.2 g/L提高到16.9 g/L,总溶剂(丙酮(acetone)、丁醇(butanol)、乙醇(ethanol),简称ABE)产量从17.8 g/L提高到24.3 g/L,葡萄糖利用率从59.4%提高到95.7%。另外,分离过程中膜的总渗透通量平均为705 g/(m2·h),丁醇分离因子平均为19.0;经渗透汽化分离,渗透液可直接进入下一步蒸馏阶段,其中丁醇和总溶剂ABE质量浓度分别为178 g/L和292 g/L,与分批发酵工艺中发酵液直接进入蒸馏塔相比,丁醇和总溶剂ABE质量浓度分别提高了10.9 倍和14.1 倍,可大大降低蒸馏能耗。

关键词: 丁醇, 渗透汽化, 原位分离, 拜氏梭菌, 发酵

Abstract:

Butanol fermentation by Clostridium beijerinckii integrated with in situ pervaporation using a
polydimethylsiloxane-polyvinylidene fluoride (PDMS-PVDF) composite membrane was carried out. The results showed
that the original glucose concentration was increased to 90 g/L for butanol fermentation integrated with pervaporation, as
compared to that (50 g/L) for the batch fermentation, and the glucose utilization was increased to 95.7% as compared to that
(59.4%) for batch fermentation. Meanwhile, butanol concentration was increased from 13.2 to 16.9 g/L, and the total amount
of solvents (acetone-butanol-ethanol, ABE) was increased from 17.8 g/L to 24.3 g/L based on the original glucose of 90 g/L.
In addition, the average total flux was 705 g/(m2·h) and separation factor of butanol was 19.0 during the butanol separation
process. The concentrations of butanol and total solvents were 178 and 292 g/L in the permeates, which were increased by
11.9 and 15.1 folds, respectively, as compared to those for batch fermentation.

Key words: butanol, pervaporation, in situ separation, Clostridium beijerinckii, fermentation

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