食品科学

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脱氢表雄酮对大鼠脂肪代谢的影响及机理

陈 迪,康 健,马海田*   

  1. 南京农业大学 农业部动物生理生化重点实验室,江苏 南京 210095
  • 出版日期:2015-09-15 发布日期:2015-09-11

Effect and Mechanism of Different Doses of Dehydroepiandrosterone on Lipid Metabolism in SD Rats

CHEN Di, KANG Jian, MA Haitian*   

  1. Key Laboratory of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China
  • Online:2015-09-15 Published:2015-09-11

摘要:

目的:以雄性Sprague-Dawley(SD)大鼠为对象,探讨日粮中添加不同剂量的脱氢表雄酮(dehydroepiandrosterone,DHEA)对大鼠脂肪代谢常规生化指标及相关基因表达的影响。方法:将60 只雄性SD大鼠随机分为对照组,DHEA低、中、高剂量组,各处理组大鼠分别灌胃0、25、50、100 mg/(kg•d)的DHEA(以体质量计,下同),连续灌胃8 周,测定大鼠血脂、肝脂水平及肝脏脂肪代谢相关基因表达量。结果:DHEA中剂量组大鼠体质量显著低于对照组(P<0.05)。中剂量的DHEA可极显著降低大鼠血清中甘油三酯和血糖含量(P<0.01),低、中剂量的DHEA可显著升高大鼠血清高密度脂蛋白胆固醇含量(P<0.05)。脂肪代谢合成相关基因分析结果表明,低、高剂量的DHEA可显著或极显著降低大鼠肝脏组织中脂肪酸合成酶(fatty acid synthase,FAS)mRNA表达水平(P<0.05或P<0.01);与对照组相比,不同剂量的DHEA均可显著或极显著降低大鼠肝脏组织中固醇调节元件结合蛋白-1(sterol regulatory element binding protein-1,SREBP-1)mRNA和肝细胞核因子-4(hepatocyte nuclear factor-4,HNF-4)mRNA表达水平(P<0.05或P<0.01),但对乙酰辅酶A羧化酶(acetylcoenzyme A carboxylase,ACC)mRNA表达水平均无显著影响(P>0.05)。脂肪代谢分解相关基因分析结果表明,不同剂量DHEA均可显著或极显著降低大鼠肝脏组织中酰基辅酶A氧化酶(acyl coenzyme A oxidase,ACO)mRNA表达水平(P<0.05或P<0.01);与对照组相比,低、高剂量的DHEA可显著或极显著降低大鼠肝脏组织中肉毒碱棕榈酰转移酶-1(carnitine palmitoyl transterase-1,LCPT-1)mRNA和过氧化物酶体增殖物激活受体α(peroxisomeproliferator activated receptor-α,PPARα)mRNA表达水平(P<0.05或P<0.01);中、高剂量的DHEA可显著或极显著降低大鼠肝脏组织中脂肪三酰甘油脂肪酶(adipose triglyceride lipase,ATGL)mRNA表达水平(P<0.05或P<0.01)。结论:长期灌胃DHEA可影响雄性大鼠脂肪代谢途径中关键因子基因的表达,最终降低大鼠体内脂肪的沉积。

关键词: 脱氢表雄酮, 脂类代谢, mRNA

Abstract:

This study aimed to investigate the effects of dehydroepiandrosterone (DHEA) on routine biochemical indicators
and the expression levels of lipid metabolism-related genes. A total of 60 male Sprague-Dawley (SD) male rats were
randomly divided into 4 groups: control group (0 mg/(kg·d) DHEA), low-dose group (25 mg/(kg·d) DHEA), moderate-dose
group (50 mg/(kg·d) DHEA) and high-dose group (100 mg/(kg·d) DHEA). The results showed that DHEA significantly
decreased body weight in moderate-dose group when compared with control group (P < 0.05). The levels of serum
triacylglycerol and glucose were highly significantly decreased in moderate-dose group (P < 0.01). The level of high-density
lipoprotein cholesterol (HDL-C) was significantly increased in high-dose group (P < 0.05). The results of quantitative realtime
polymerase chain reaction (qRT-PCR) showed that mRNA expression level of fatty acid synthase (FAS) was significantly
decreased in moderate-dose group (P < 0.05 or P < 0.01) when compared with control group. The mRNA expression levels
of sterol regulatory element binding protein-1 (SREBP-1) and hepatocyte nuclear factor-4 (HNF-4) were significantly
decreased in all the DHEA groups (P < 0.05 or P < 0.01). However, the mRNA expression level of acetyl coenzyme A
carboxylase (ACC) was not significantly changed (P > 0.05). As for the adipolysis-related genes, the mRNA expression
levels of acyl coenzyme A oxidase (ACO) were significantly decreased in all the DHEA groups (P < 0.05 or P < 0.01).
The mRNA expression levels of carnitine palmitoyl transterase-1 (LCPT-1) and peroxisome proliferator activated receptor-α
(PPARα) were significantly decreased in both low- and high-dose groups (P < 0.05 or P < 0.01). The mRNA expression levels of
adipose triglyceride lipase (ATGL) were significantly decreased in both moderate- and high-dose groups (P < 0.05 or P < 0.01). These
findings indicate that DHEA affects the key factors affecting lipid metabolism, resulting in decreased fat deposition in male rats.

Key words: dehydroepiandrosterone (DHEA), lipid metabolism, mRNA

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