响应面试验优化超临界CO2酯交换法制备低能SLS型结构脂质工艺

doi:10.7506/spkx1002-6630-201520012

食品科学

响应面试验优化超临界CO₂酯交换法制备低能SLS型结构脂质工艺

张家明，许多现，李相昕，齐晓芬，张旭，于殿宇*

东北农业大学食品学院，黑龙江哈尔滨 150030

出版日期:2015-10-25 发布日期:2015-10-20
通讯作者: 于殿宇
基金资助:
东北农业大学大学生SIPT计划项目（201410224036）

Process Optimization for Synthesis of Low-Energy SLS-Type Structured Lipid by Transesterification in Supercritical CO₂ State Using Response Surface Methodology

ZHANG Jiaming, XU Duoxian, LI Xiangxin, QI Xiaofen, ZHANG Xu, YU Dianyu

School of Food Science and Technology, Northeast Agricultural University, Harbin 150030, China

Online:2015-10-25 Published:2015-10-20
Contact: YU Dianyu

摘要/Abstract

摘要：

在超临界CO2状态下，采用脂肪酶RMIM催化乙酸乙酯与一级大豆油反应制备SLS型结构脂质，分别应用单因素试验和响应面试验考察酶用量、反应压力、温度和时间对乙酸乙酯转化率的影响。结果表明，最佳工艺条件为酶用量2.9%、反应时间13.1 h、反应压力9.4 MPa、反应温度49.0 ℃，此条件下乙酸乙酯的转化率可达到36.1%，经纯化后获得纯度为90.3%、热值为22.35 kJ/g的SLS型结构脂质，与普通大豆油相比，热值降低了42%。

关键词: 超临界CO2, 脂肪酶, 结构脂质, 乙酸乙酯转化率

Abstract:

In the supercritical CO2 state, the synthesis of lipid with SLS-type structure from the Rhizomucor miehei lipase
(Lipozyme RMIM)-catalyzed reaction between ethyl acetate and first-grade soybean oil was investigated. The effects of
enzyme concentration, reaction pressure, temperature and time on the conversion rate of ethyl acetate were examined by the
combined use of single factor design and response surface methodology. The results showed that the optimal parameters
for preparing SLS-type structured lipid were determined as follows: in the presence of 2.9% lipase RMIM, reaction at
49.0 ℃and 9.4 MPa for 13.1 h. Under these conditions, the conversion rate of ethyl acetate reached 36.1% and after
purification of the final product, a purity of 90.3% and a caloricity of 22.35 kJ/g (reduced by 42% as compared with that of
ordinary soybean oil) were obtained.

Key words: supercritical CO2, lipase, structured lipid, ethyl acetate conversion rate

中图分类号:

TS224.8

张家明，许多现，李相昕，齐晓芬，张旭，于殿宇. 响应面试验优化超临界CO₂酯交换法制备低能SLS型结构脂质工艺[J]. 食品科学, doi: 10.7506/spkx1002-6630-201520012.

ZHANG Jiaming, XU Duoxian, LI Xiangxin, QI Xiaofen, ZHANG Xu, YU Dianyu. Process Optimization for Synthesis of Low-Energy SLS-Type Structured Lipid by Transesterification in Supercritical CO₂ State Using Response Surface Methodology[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201520012.

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响应面试验优化超临界CO₂酯交换法制备低能SLS型结构脂质工艺

Process Optimization for Synthesis of Low-Energy SLS-Type Structured Lipid by Transesterification in Supercritical CO₂ State Using Response Surface Methodology

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