食品科学

• 基础研究 • 上一篇    下一篇

橘皮素的提取分离、改性及对人体肝星状细胞LX-2的抑制作用

徐兰英1,赵 辉2,张 婷1,吴 伦1,龙 涛1,*,李士明1,*   

  1. 1.黄冈师范学院化工学院,催化材料制备及应用湖北省重点实验室,湖北 黄冈 438000;
    2.天津商业大学生物技术与食品科学学院,天津市食品生物技术重点实验室,天津 300134
  • 出版日期:2015-12-15 发布日期:2015-12-24

Isolation and 5-Demethylation of Tangertin from Chen-Pi and Their Inhibitory Effect on Hepatic Stellate Cell LX-2

XU Lanying1, ZHAO Hui2, ZHANG Ting1, WU Lun1, LONG Tao1,*, LI Shiming1,*   

  1. 1. Hubei Key Laboratory for Processing and Application of Catalytic Materials, College of Chemical Technology, Huanggang Normal University, Huanggang 438000, China; 2. Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China
  • Online:2015-12-15 Published:2015-12-24

摘要:

通过分离纯化及改性得到橘皮素和5-去甲氧基橘皮素,并研究其对人体肝星状细胞株LX-2的抑制作用。通过无水乙醇提取、氯仿萃取从陈皮中得到多甲基黄酮混合物,再以不同浓度的乙酸乙酯和石油醚的混合液为洗脱剂,采用硅胶柱层析分离纯化多甲基黄酮混合物,得到橘皮素纯品。将橘皮素置于盐酸乙醇溶液中回流,对所得产物进行纯化即可得5-去甲氧基橘皮素,采用质谱和核磁共振谱进行结构确证。利用人体肝星状细胞株LX-2细胞作为工具细胞,研究其生物活性。结果表明,5-去甲氧基橘皮素在12.5 μmol/L条件下对人肝星状细胞株LX-2的抑制率即可达到50%,而橘皮素需要在近150 μmol/L条件下才能达到50%的抑制率,即改性后的橘皮素对人体肝细胞的抑制有效率增加了10 倍以上。

关键词: 橘皮素, 5-去甲氧基橘皮素, 多甲氧基黄酮, 肝星状细胞LX-2

Abstract:

An efficient and large-scale isolation method for tangeretin from Chen-Pi (aged tangerine peels) has been
developed successfully. The transformation of tangeretin to 5-demethyltangeretin was performed in acidified alcohol solution
with high conversion efficiency. The structures of both isolated tangeretin and 5-demethyltangeretin were confirmed by MS
and 1H NMR. Both compounds exhibited strong inhibitory activity against LX-2 human hepatic stellate cells, with an IC50
of 150 μmol/L for tangeretin and 12.5 μmol/L for 5-demethyltangeretin, indicating the increased potency via the conversion
from tangeretin to 5-demethyltangeretin.

Key words: tangeretin, 5-demethyltangeretin, polymethoxyflavones, hepatic stellate cell LX-2

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