食品科学

• 基础研究 • 上一篇    下一篇

烘焙条件对小粒黑豆中抗氧化组分及抗氧化活性的影响

乔丽华,王美霞,王艺静,杜双奎*   

  1. 西北农林科技大学食品科学与工程学院,陕西 杨凌 712100
  • 出版日期:2016-01-15 发布日期:2016-01-15

Influence of Roasting on Antioxidant Substances and Antioxidant Activity of Small Black Soybean

QIAO Lihua, WANG Meixia, WANG Yijing, DU Shuangkui*   

  1. College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China
  • Online:2016-01-15 Published:2016-01-15

摘要:

小粒黑豆的抗氧化作用与其所含有的酚类、花色苷的含量等密切相关,而这些物质在加工过程中会受温度和时间的影响而发生变化。实验以盐池黑豆、府谷小黑豆两种小粒黑豆为材料,分析烘焙处理对其总酚、花色苷含量、总抗氧化能力及1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基、羟自由基(·OH)、超氧阴离子自由基(O2-·)清除能力的影响。结果表明,烘焙对小粒黑豆的抗氧化活性有显著影响,200 ℃条件下烘焙后的总酚含量显著高于100、150 ℃,150 ℃的总酚含量最低;总花色苷含量随着烘焙温度的升高、处理时间的延长而显著减少;·OH清除作用随着烘焙温度的升高而显著降低;烘焙样品的O2-·清除作用显著低于未烘焙样品(P<0.05)。盐池黑豆、府谷小黑豆的总酚含量、总抗氧化能力以及DPPH自由基、O2-·的清除作用在150 ℃时最低。200 ℃条件下烘焙30 min,盐池黑豆的总酚含量、总抗氧化能力、DPPH自由基清除率最高;府谷小黑豆在200 ℃条件下烘焙40 min,其总酚含量最高,而100 ℃条件下烘焙20 min时的花色苷含量、总抗氧化能力、DPPH自由基清除率最高。

关键词: 小粒黑大豆, 烘焙, 抗氧化性, 总酚, 花色苷

Abstract:

The antioxidant activity of small black soybean is closely related to its contents of phenolic compounds and
anthocyanins; however, these substances are easily affected by different thermal processing conditions. Roasting condition
has a significant effect on the antioxidant activity of small black soybean. In this study, the effect of roasting temperature and
time on the total phenolic, anthocyanins contents and antioxidant activity of small black soybean from Yanchi and Fugu was
investigated. Our results indicated that the small soybean roasted at 150 ℃ showed the lowest content of total phenols, while
that roasted at 200 ℃ had the highest content of total phenols. The content of total anthocyanins markedly declined with
increasing roasting temperature and time. The hydroxyl radical scavenging activity of small black soybean became lower
with increasing roasting temperature, and the roasted sample had superoxide anion radical scavenging activity significantly
lower than that of the unroasted one (P < 0.05). The roasted small soybean at 150 ℃ irrespective of the geographical origin
exhibited the lowest content of total phenols, total antioxidant capacity (ferric-reducing ability of plasma, FRAP), and radical
scavenging activity against DPPH and superoxide anion radicals. Moreover, the Yanchi small soybean roasted at 200 ℃ for
30 min showed the highest total phenol content and DPPH radical scavenging activity, while the small black soybean from
Fugu roasted at 200 ℃ for 40 min exhibited the highest total phenol content and had the highest anthocyanint content, FRAP
value and DPPH radical scavenging activity when roasted at 100 ℃ for 20 min.

Key words: small black soybean, roasting, antioxidant activity, total phenols, anthocyanins

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