食品科学

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异甘草素对3T3-L1前脂肪细胞分化的抑制作用

邱理红1,王占洋1,李雅娟1,佀营营2,周 慧1,韩 欢2,许 波2,李 刚2,谢建新1,李 忌2,王振华2,*   

  1. 1.石河子大学药学院,新疆 石河子 832005;2.烟台大学生命科学学院,线粒体与健康衰老研究中心,山东 烟台 264005
  • 出版日期:2016-01-15 发布日期:2016-01-15

Isoliquiritigenin Inhibits the Differentiation of 3T3-L1 Preadipocytes

QIU Lihong1, WANG Zhanyang1, LI Yajuan1, SI Yingying2, ZHOU Hui1, HAN Huan2, XU Bo2, LI Gang2, XIE Jianxin1, LI Ji2, WANG Zhenhua2,*   

  1. 1. School of Pharmacy, Shihezi University, Shihezi 832005, China;
    2. Center of Mitochondria and Healthy Aging, College of Life Sciences, Yantai University, Yantai 264005, China
  • Online:2016-01-15 Published:2016-01-15

摘要:

目的:考察异甘草素对3T3-L1前脂肪细胞分化的影响,探究其作用机制。方法:采用3T3-L1前脂肪细胞,利用磺酰罗丹明B(sulforhodamine B,SRB)法检测异甘草素对3T3-L1前脂肪细胞增殖的影响,采用传统的鸡尾酒法诱导3T3-L1细胞分化为脂肪细胞,利用油红O染色法检测分化细胞脂滴形成;采用实时荧光定量逆转录聚合酶链式反应方法,检测细胞CCAAT增强子结合蛋白(CCAAT-enhancer-binding proteins,C/EBP)亚型C/EBPα、C/EBPβ、脂肪酸合成酶(fatty acid synthetase,FAS)、脂肪分化相关蛋白adipophilin、过氧化物酶体增殖物激活受体γ(peroxisome proliferators activated receptor γ,PPARγ)、腺苷酸活化蛋白激酶(adenosine monophosphateactivated protein kinase,AMPK)的mRNA表达水平,Western blotting法检测AMPK蛋白磷酸化水平。结果:异甘草素可浓度依赖性地抑制3T3-L1细胞增殖,并明显抑制分化细胞内脂滴形成,同时抑制了细胞分化相关基因C/EBPα、C/EBPβ、FAS、adipophilin、PPARγ mRNA的表达,AMPK mRNA的表达未受影响,但异甘草素处理明显上调了AMPK蛋白的磷酸化水平。结论:异甘草素可抑制3T3-L1前脂肪细胞向脂肪细胞分化,其机制可能与活化AMPK信号通路相关。

关键词: 异甘草素, 3T3-L1前脂肪细胞, 分化, 脂质生成, 腺苷酸活化蛋白激酶

Abstract:

Objective: To investigate the potential effects and underlying mechanisms of isoliquiritigenin on the
differentiation of 3T3-L1 preadipocytes. Methods: The SRB assay was used to evaluate the effects of isoliquiritigenin on the
proliferation of 3T3-L1 preadipocytes. The differentiation of 3T3-L1 cells was induced by cocktail reagents. The formation
of lipid droplets in the differentiated adipocytes was observed after oil red O staining and quantified by colorimetry. The
expression of CCAAT-enhancer-binding proteins (C/EBP)-α and-β, fatty acid synthetase (FAS), adipophilin, peroxisome
proliferators activated receptor γ (PPARγ) and adenosine monophosphate activated protein kinase (AMPK) genes was
measured by quantity real-time reverse transcript polymerase chain reaction (qRT-PCR). The phosphorylation of AMPK
protein was detected by Western blotting. Results: Isoliquiritigenin inhibited the proliferation of 3T3-L1 preadipocytes in
a concentration-dependent manner. The isoliquiritigenin treatment significantly decreased the formation of lipid droplets
in differentiated adipocytes. Furthermore, isoliquiritigenin down-regulated the mRNA expression of C/EBPα, C/EBPβ,
FAS, adipophilin, and PPARγ genes. On the other hand, isoliquiritigenin had no obvious effects on the mRNA expression of
AMPK gene, but up-regulated the phosphorylation of AMPK. Conclusion: Isoliquiritigenin could inhibit the proliferation
and differentiation of 3T3-L1 preadipocytes due to the activation of AMPK signal pathway.

Key words: isoliquiritigenin, 3T3-L1 preadipocytes, differentiation, lipogenesis, adenosine monophosphate activated protein kinase (AMPK)

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