藏灵菇源克鲁维酵母菌M3调节大鼠肠道菌群平衡的研究

doi:10.7506/spkx1002-6630-201605035

食品科学

藏灵菇源克鲁维酵母菌M3调节大鼠肠道菌群平衡的研究

董牧群1，熊利霞1，谢远红1，张红星1，刘慧1,*，贾慧1，连正兴2

1.北京农学院食品科学与工程学院，微生态制剂关键技术开发北京市工程实验室，食品质量与安全北京实验室，北京 102206；2.中国农业大学动物科技学院，北京 100091

出版日期:2016-03-15 发布日期:2016-03-17

Regulatory Effect of Kluyveromyces marxianus M3 Isolated from Tibetan Kefir on the Intestinal Flora Balance in Rats

DONG Muqun1, XIONG Lixia1, XIE Yuanhong1, ZHANG Hongxing1, LIU Hui1,*, JIA Hui1, LIAN Zhengxing2

1. Beijing Engineering Laboratory of Key Technology Development of Microecologics, Beijing Laboratory of Food Quality and Safety, College of Food Science and Engineering, Beijing University of Agriculture, Beijing 102206, China; 2. College of Animal Science and Technology, China Agricultural University, Beijing 100091, China

Online:2016-03-15 Published:2016-03-17

摘要/Abstract

摘要：

利用从藏灵菇中筛选出的发酵性能优良且产胆盐水解酶的马克斯克鲁维酵母菌M3菌株，制备冻干菌粉作为受试物进行动物实验，探讨其对预防性高胆固醇血症大鼠肠道菌群平衡的作用。采用预防高胆固醇血症大鼠模型，将40 只无特定病原体（specific pathogen free，SPF）级3 周龄的大鼠分为高脂模型组、低剂量、中剂量和高剂量实验组进行实验。实验组分别灌胃低、中、高剂量的受试物，高脂模型组灌胃蒸馏水，连续7 周。结果表明，前5 周4 组大鼠粪便中乳酸杆菌（lactobacilli）、沙门氏菌（Salmonella）、产气荚膜梭菌（Clostridium perfringens）的数量处于平缓变化状态，且3 个实验组与高脂模型组相比无显著性差异；5 周时，3 个实验组大鼠粪便中乳杆菌的数量极显著高于高脂模型组（P＜0.01），沙门氏菌的数量极显著低于高脂模型组（P＜0.01），产气荚膜梭菌的数量显著低于高脂模型组（P＜0.05）；其中低剂量组的乳杆菌数量最高而沙门氏菌和产气荚膜梭菌数量最低，说明低剂量实验组优于中、高剂量实验组。显示藏灵菇源克鲁维酵母菌M3能够调节高胆固醇血症大鼠肠道有益菌的生长，并抑制有害菌的繁殖，具有良好的调节肠道菌群平衡的效果。

关键词: 藏灵菇, 马克斯克鲁维酵母菌, 动物实验, 肠道菌群平衡

Abstract:

To evaluate the effects of Kluyveromyces marxianus M3, with good fermentation performance and high ability to
produce bile salt hydrolase, isolated from Tibetan kefir on the intestinal flora balance in experimental hypercholesterolemic
rats, 40 rats (43 weeks old) were divided into 4 groups and fed a high fat diet supplemented with low, medium and high
dose of freeze-dried K. marxianus M3 powder for a 7-week experimental period. According to our results, in the first five
weeks, the numbers of 3 kinds of bacteria in rat feces gradually changed, and there was no significant difference between the
three experimental groups and the high-fat model group; at the fifth week, the preventive hypercholesterolemia model was
established successfully, the number of Lactobacillus in the three experimental groups was significantly higher than that in
the high-fat model group (P < 0.01), while the number of Salmonella (P < 0.01) and Clostridium perfringens (P < 0.05) was
significantly lower than in the high-fat model; among all experimental groups, the number of Lactobacillus in the low dose
group was the highest while Salmonella and Clostridium perfringens were the lowest, showing that the effect of low dose
was better than the medium and high doses. These results demonstrated that K. marxianus M3 could promote the growth
of intestinal beneficial bacteria and inhibit the breeding of harmful bacteria in the hypercholesterolemic rats, having a good
effect on the intestinal flora balance.

Key words: Tibetan kefir, Kluyveromyces marxianus, animal experiment, intestinal flora balance

中图分类号:

Q93.337

董牧群，熊利霞，谢远红，张红星，刘慧，贾慧，连正兴. 藏灵菇源克鲁维酵母菌M3调节大鼠肠道菌群平衡的研究[J]. 食品科学, doi: 10.7506/spkx1002-6630-201605035.

DONG Muqun, XIONG Lixia, XIE Yuanhong, ZHANG Hongxing, LIU Hui, JIA Hui, LIAN Zhengxing. Regulatory Effect of Kluyveromyces marxianus M3 Isolated from Tibetan Kefir on the Intestinal Flora Balance in Rats[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201605035.

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藏灵菇源克鲁维酵母菌M3调节大鼠肠道菌群平衡的研究

Regulatory Effect of Kluyveromyces marxianus M3 Isolated from Tibetan Kefir on the Intestinal Flora Balance in Rats

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