食品科学

• 工艺技术 • 上一篇    下一篇

超滤分离茅岩莓黄酮工艺优化及其抑菌作用

张艳梅,唐浩国*,焦润玲,杨同香,向进乐,刘 琼   

  1. 河南科技大学食品与生物工程学院,河南 洛阳 471023
  • 出版日期:2016-03-25 发布日期:2016-03-18

Optimization of Ultrafiltration Separation of Flavonoid from Ampelopsis grossedentata and Its Bacteriostatic Activivity

ZHANG Yanmei, TANG Haoguo*, JIAO Runling, YANG Tongxiang, XIANG Jinle, LIU Qiong   

  1. College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China
  • Online:2016-03-25 Published:2016-03-18

摘要:

采用超滤技术对茅岩莓黄酮提取液进行分离,以膜通量和黄酮纯度为评价指标,采用单因素试验和正交试验研究了超滤时间、料液质量浓度、料液温度、超滤压力对超滤效果的影响,确定最佳的分离工艺参数,并研究含有60%黄酮的茅岩莓提取物的抑菌作用。结果表明,在料液质量浓度0.5 mg/mL、料液温度35 ℃、超滤压力0.2 MPa条件下,初始膜通量为0.215 mL/(cm2·min),黄酮纯度由32.7%提高到60.1%。超滤后的黄酮提取物对4 种细菌均有明显的抑制作用,其对4 种细菌最小抑菌浓度值分别为沙门氏菌0.75 mg/mL、绿脓杆菌1.50 mg/mL、金黄色葡萄球菌1.50 mg/mL、大肠杆菌3.00 mg/mL。其对4 种细菌最低杀菌浓度值分别为沙门氏菌0.75 mg/mL、绿脓杆菌3.00 mg/mL、金黄色葡萄球菌3.00 mg/mL、大肠杆菌3.00 mg/mL。

关键词: 茅岩莓黄酮, 超滤, 膜通量, 纯度, 抑菌

Abstract:

The separation of flavonoid from the crude extract of Ampelopsis grossedentata (FAG) by ultrafiltraion was
optimized using single factor and orthogonal experiments. The independent variables were ultrafiltration time, feed
concentration, temperature and pressure, and the responses were membrane flux and flavonoid purity. Furthermore, the
bacteriostatic activity of FAG containing 60% flavonoid was studied. The results showed that when the ultrafiltration
of the sample containing 0.5 mg/mL flavonoid at 35 ℃ was conducted at 0.2 MPa, the initial membrane flux was
0.215 mL/(cm2·min), yielding an increase in FAG purity from 32.7% to 60.1%. After ultrafiltration, FAG exhibited obvious
inhibitory effects on 4 kinds of bacteria with minimal inhibitory concentration (MIC) values of 0.75, 1.50, 1.50 and 3.00 mg/mL
and minimal bactericidal concentration (MBC) values of 0.75, 3.00, 3.00 and 3.00 mg/mL for Salmonella, Pseudomonas
aeruginosa, Staphylococcus aureus, and Escherichia coli, respectively.

Key words: flavonoid from Ampelopsis grossedentata (FAG), ultrafiltration, membrane flux, purity, bacteriostasis

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