食品科学

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高效液相色谱法同时测定紫花地丁中6 种活性成分

高 意,周光明*,张彩虹,于 璐,陈军华,秦红英   

  1. 发光与实时分析教育部重点实验室,西南大学化学化工学院,重庆 400715
  • 出版日期:2016-03-25 发布日期:2016-03-18

Simultaneous Determination of Six Active Components in Viola yedoensis Makino by HPLC

GAO Yi, ZHOU Guangming*, ZHANG Caihong, YU Lu, CHEN Junhua, QIN Hongying   

  1. Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering,Southwest University, Chongqing 400715, China
  • Online:2016-03-25 Published:2016-03-18

摘要:

目的:建立超声辅助萃取-高效液相色谱法分离紫花地丁中秦皮乙素、咖啡酸、芦丁、柚皮素、木犀草素和芹菜素6 种活性成分及其含量测定的方法。方法:采用Phenomenex C18色谱柱(150 mm×4.6 mm,5 μm)分离6 种成分;流动相为甲醇-0.1%醋酸溶液,梯度洗脱;流速0.8 mL/min,紫外检测波长340 nm,柱温35 ℃。结果:6 种成分在15 min内均达到基线分离,线性关系良好(r≥0.999 1,n=6),平均回收率均在102.13%~104.68%(相对标准偏差小于1.20%,n=3)。结论:本实验采用超声波法提取,并对提取条件进行探讨,得出最佳条件为超声时间30 min、超声功率120 W、固液比1∶100(g/mL)、甲醇体积分数60%。同时该研究表明该方法简单、快速、经济、可靠,可用于紫花地丁的品质监控。

关键词: 高效液相色谱, 紫花地丁, 超声萃取, 活性成分, 含量测定

Abstract:

Objective: To establish an analytical method for the simultaneous separation and determination of cichorigenin,
caffeic acid, rutin, naringenin, luteolin and kaempferol in Viola yedoensis Makino by ultrasonic-assisted extraction and
high performance liquid chromatography (HPLC). Methods: The separation of six active components was performed on
a Phenomenex C18 column (150 mm × 4.6 mm, 5 μm) with stepwise gradient elution. The mobile phase was a mixture of
methanol and 0.1% acetic acid at a flow rate of 0.8 mL/min; the UV detection wavelength was 340 nm and the column
temperature was set at 35 ℃. Results: Baseline separation of cichorigenin, caffeic acid, rutin, naringenin, luteolin and
kaempferol was achieved within 15 min. The calibration curves of the six components showed good linear relationship (r ≥
0.999 1, n = 6). The average recoveries of spiked samples were in the range of 102.13%–104.68% (RSD < 1.20%, n = 3).
Conclusions: The optimal conditions for ultrasonic-assisted extraction were determined as follows: ultrasonic time, 30 min;
ultrasonic power, 120 W; solid/liquid ratio, 1:100 (g/mL); and extraction solvent, 60% methanol. This study also showed that
the HPLC method was simple, rapid, economic and reliable and could be used for quality control of Viola yedoensis.

Key words: high performance liquid chromatography (HPLC), Viola yedoensis Makino, ultrasonic-assisted extraction; active components, quantitation

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