食品科学

• 分析检测 • 上一篇    下一篇

HPLC-PDA法测定明日叶不同部位中的4-羟基德里辛和黄色当归醇含量

李亚丽1,2,罗玉兰2,刘建华3,吴金鸿4,钟耀广1,*,王正武4,*   

  1. 1.上海海洋大学食品学院,上海 201306;2.上海市园林科学研究所,上海 200232;
    3.宜宾学院化学与化工学院,四川 宜宾 644000;4.上海交通大学农业与生物学院,上海 200240
  • 出版日期:2016-03-25 发布日期:2016-03-18

Determination of 4-Hydroxyderricin and Xanthoangelol in Different Parts of Angelica keiskei by HPLC-PDA

LI Yali1,2, LUO Yulan2, LIU Jianhua3, WU Jinhong4, ZHONG Yaoguang1,*, WANG Zhengwu4,*   

  1. 1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China;
    2. Shanghai Institute of Landscape Gardening Science, Shanghai 200232, China;
    3. College of Chemistry and Chemical Engineering, Yibin University, Yibin 644000, China;
    4. School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China
  • Online:2016-03-25 Published:2016-03-18

摘要:

采用高效液相色谱配备光电二极管阵列检测器法测定明日叶根、茎和叶中4 - 羟基德里辛(4-hydroxyderricin,4-HD)和黄色当归醇(xanthoangelol,XAG)的含量。样品分析选用Halo C18色谱柱(4.6 mm×150 mm,2.7 μm),流动相为0.1%甲酸(A)-甲醇(B)溶液,梯度洗脱,流速为0.3 mL/min,检测波长为370 nm,柱温为40 ℃。4-HD、XAG在0.945~18.900、1.048~20.960 μg/mL质量浓度范围内线性关系良好,样品含量的相对标准偏差分别为3.13%、2.52%,方法的重复性好,平均回收率分别为103.70%、103.05%。经检测明日叶中4-HD的分布规律为:茎(149.16 μg/g)>根(122.42 μg/g)>叶(1.24 μg/g);XAG的分布规律为:根(144.28 μg/g)>茎(75.17 μg/g)>叶(1.21 μg/g)。4-HD和XAG在根和茎中的分布显著高于其在叶中的分布。

关键词: 明日叶, 4-羟基德里辛, 黄色当归醇, 高效液相色谱配备光电二极管阵列检测器

Abstract:

In this study, the contents of 4-hydroxyderricin (4-HD) and xanthoangelol (XAG) in roots, stems and leaves of
Angelica keiskei were determined using high performance liquid chromatography with photo-diode array (HPLC-PDA)
detector. The samples were separated on a Halo C18-column (4.6 mm × 150 mm, 2.7 μm), gradiently eluted with 0.1% formic
acid in water (A) and methanol (B) at a flow rate of 0.3 mL/min and detected at 370 nm. The column temperature was set
at 40 ℃. Good linear relationships between peak area and injection amount of 4-HD and XAG were established within the
range of 0.945–18.900 and 1.048–20.960 μg/mL, respectively. The relative standard deviations (RSDs) of peak area for the
two compounds were 3.13% and 2.52%, respectively, suggesting good reproducibility of the developed method. The average
recoveries of the spiked compounds were 103.70%, and 103.05%, respectively. The results showed that the distribution
of 4-HD in different parts of Angelica keiskei was ranked in decreasing order as follows: stems (149.16 μg/g) > roots
(122.42 μg/g) > leaves (1.24 μg/g), while the order of XAG content was as follows: roots (144.28 μg/g) > stems (75.17 μg/g) >
leaves (1.21 μg/g). The contents of 4-HD and XAG in roots and stems of Angelica keiskei were significantly higher than in leaves.

Key words: Angelica keiskei, 4-hydroxyderricin (4-HD), xanthoangelol (XAG), high performance liquid chromatography with photo-diode array (HPLC-PDA)

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