食品科学

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两株素食者肠道菌产β-葡萄糖苷酶考察及产酶条件优化

李笑梅,马慧玲   

  1. 哈尔滨商业大学 黑龙江省高校食品科学与工程重点实验室,黑龙江 哈尔滨 150076
  • 出版日期:2016-04-15 发布日期:2016-04-13

Comparison of Two β-Glucosidase-Producing Strains from the Intestinal Tract of Vegetarians and Optimizaiton of Fermentation Conditions for β-Glucosidase Production

LI Xiaomei, MA Huiling   

  1. Key Laboratory of Food Science and Engineering, Harbin University of Commerce, Harbin 150076, China
  • Online:2016-04-15 Published:2016-04-13

摘要:

采用对硝基苯基-β-D-葡萄糖苷法,与黑曲霉相比较考察素食者肠道菌LJ-G1、LJ-Q2产β-葡萄糖苷酶能力,再经单因素、响应面试验对菌种的产酶条件进行优化。结果表明:LJ-G1、LJ-Q2菌株均能产β-葡萄糖苷酶,且LJ-Q2产酶能力高于LJ-G1,与黑曲霉相比较,在发酵时间短于64 h时LJ-G1、LJ-Q2产酶能力高于黑曲霉;LJ-Q2菌种的最优产酶条件为:培养基pH 8.0、培养温度38 ℃、培养时间38 h。在最优条件下进行验证实验,酶活力达到1.70 IU/mL。加入大豆异黄酮原料进行发酵培养,得到游离大豆异黄酮转化率为39.4%。

关键词: 素食者, 肠道菌, &beta, -葡萄糖苷酶, 产酶条件

Abstract:

The β-glucosidase-producing capacity of strains LJ-G1 and LJ-Q2 isolated from the feces of vegetarians was
measured using 4-nitrophenol-β-D-glucoside (pNPG) as substrate and compared with that of Aspergillus niger. The
fermentation conditions for β-glucosidase production by the selected strain were optimized using combination of single factor
method and response surface methodology. The results showed that both intestinal strains were able to produce β-glucosidase,
and LJ-Q2 had higher β-glucosidase-producing capacity than LJ-G1. LJ-G1 and LJ-Q2 had higher β-glucosidase-producing
capacity than Aspergillus niger in 64 h of fermentation. The optimal fermentation conditions for β-glucosidase production
were determined as follows: culture medium pH, 8.0; temperature, 38 ℃; and time, 38 h. The experimental value of
β-glucosidase activity produced under the optimized conditions was 1.70 IU/mL. The fermentation process could convert
39.4% of soybean isoflavones into aglycones.

Key words: vegetarians, intestinal bacteria, β-glucosidase, fermentation conditions for β-glucosidase production

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