食品科学

• 基础研究 • 上一篇    下一篇

紫薯花青素的提取及其在VC含量测定中的应用

郭彩华,卢珍华,伍 菱,陈昭华,陈庆绸,熊何健*   

  1. 集美大学食品与生物工程学院,福建 厦门 361021
  • 出版日期:2016-05-15 发布日期:2016-05-18

Anthocyanins from Purple Sweet Potato (Ipomoea batatas) and Its Application in VC Quantification

GUO Caihua, LU Zhenhua, WU Ling, CHEN Zhaohua, CHEN Qingchou, XIONG Hejian*   

  1. College of Food and Biological Engineering, Jimei University, Xiamen 361021, China
  • Online:2016-05-15 Published:2016-05-18

摘要:

从紫薯中提取花青素,该花青素在530 nm波长处有特征吸收峰,分子质量范围在850~1 020 u之间。利用紫薯花青素替代GB/T 6195—1986《水果、蔬菜维生素C含量测定法(2,6-二氯靛酚测定法)》测定夏橙和西红柿中VC含量。结果表明:夏橙和西红柿中VC的含量分别为(25.33±0.34)、(20.41±0.32) mg/100 g;紫薯花青素法的加标回收率为95.4%~98.6%,相对标准偏差(relative standard deviation,RSD)为1.18%~1.22%;2,6-二氯靛酚法的加标回收率为96.5%~98.7%,RSD为0.86%~0.88%。建立的利用紫薯花青素替代GB/T 6195—1986中2,6-二氯靛酚测定水果、蔬菜中VC含量的方法具有安全、廉价的特点,其准确度与2,6-二氯靛酚法滴定法相当。

关键词: 紫薯, 花青素, GB/T 6195&mdash, 1986, 2, 6-二氯靛酚, VC

Abstract:

Anthocyanins were extracted from purple sweet potato (Ipomoea batatas) using an environmentally friendly
process. The anthocyanins exhibited characteristic absorption peak at 530 nm, having a molecular weight in the range of
850?1 020 u. The content of vitamin C in Valencia orange and tomato was determined by a new titration method using the
anthocyanins extracted from purple sweet potato in place of 2,6-dichloroindophenol as described in the Chinese national
standard GB/T 6195-1986. The results showed that vitamin C was (25.33 ± 0.34) mg/100 g in Valencia orange and (20.41 ±
0.32) mg/100 g in tomato. The spiked recoveries of VC with purple sweet potato anthocyanins ranged from 95.4% to 98.6%,
with relative standard deviation (RSD) of 1.18%?1.22%, while those with 2,6-dichloroindophenol titration were between
96.5% and 98.7% with RSD of 0.86%?0.88%. The anthocyanin titration method was safe and low-cost with an accuracy
equivalent to that of 2,6-dichloro-indophenol titration.

Key words: Ipomoea batatas, anthocyanins, GB/T 6195-1986, 2,6-dichloroindophenol, vitamin C

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