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超声波-生物酶法提取锁阳多糖工艺优化及其抗肿瘤活性

张慧瑛1,罗光宏2,*,郝军元1,杨生辉2,崔 伟1,张 杰1   

  1. 1.河西学院农业与生物技术学院,甘肃 张掖 734000;2.河西学院凯源生物技术开发中心,甘肃 张掖 734000
  • 出版日期:2016-06-25 发布日期:2016-06-29
  • 通讯作者: 罗光宏
  • 基金资助:

    甘肃省青年科技研究基金项目(1107RJYG012);甘肃省科技支撑计划项目(1304FKCG103)

Optimization of Ultrasonic-Assisted Enzymatic Extraction of Polysaccharides from Cynomorium songaricum and Their Antitumor Activity

ZHANG Huiying, LUO Guanghong, HAO Junyuan, YANG Shenghui, CUI Wei, ZHANG Jie   

  1. 1. College of Agriculture and Biotechnology, Hexi University, Zhangye 734000, China;
    2. Kaiyuan Biology Technology Development Centre, Hexi University, Zhangye 734000, China
  • Online:2016-06-25 Published:2016-06-29
  • Contact: LUO Guanghong

摘要:

为了获得较高的锁阳多糖得率,以河西锁阳为原料,采用超声波协同生物酶技术进行锁阳多糖提取工艺及活性的研究,选用单因素试验探索料液比、超声时间、超声功率及纤维素酶加酶量、酶解时间、酶解温度、pH值对锁阳多糖得率的影响,在单因素试验的基础上,采用正交试验对工艺条件进行优化,并采用四甲基噻唑蓝(methlthiazoletrazolium,MTT)法评价锁阳多糖对HeLa细胞的抗肿瘤活性。结果表明,锁阳多糖超声波-纤维素酶法提取最佳工艺为:料液比1∶10(g/mL)、超声功率300 W、酶解温度60 ℃、超声时间10 min、加酶量1.8%、酶解时间90 min、pH 5.5。最优条件下锁阳多糖得率达3.01%。MTT实验结果表明,提取多糖对HeLa细胞具有明显的抗肿瘤活性。

关键词: 锁阳, 多糖, 提取, 超声波-纤维素酶, 生物活性

Abstract:

The ultrasonic-assisted enzymatic extraction of polysaccharides from the dried fleshy stem of Cynomorium
songaricum grown in the Hexi Corridor region, Gansu province was optimized by one-factor-at-a-time and orthogonal
array design methods for improved yield of polysaccharides. The effects of the ratio of material to water, ultrasonic treatment
time, ultrasonic power, cellulase dosage, hydrolysis temperature, time, and pH on the extraction yield of polysaccharides
were investigated. The inhibitory effects of the extracted polysaccharides on the proliferation of human cervical cancer HeLa
cells in vitro were studied. The optimal extraction conditions obtained were as follows: material to water ratio, 1:10 (g/mL);
ultrasonic treatment time, 10 min; ultrasonic power, 300 W; enzyme dosage, 1.8%; hydrolysis temperature, 60 ℃; hydrolysis time,
90 min; and initial pH, 5.5. Under these conditions, the maximum yield of polysaccharides of 3.01% was obtained. MTT assay
results showed that the polysaccharide had obvious anti-tumor activity against human cervical cancer HeLa cells.

Key words: Cynomorium songaricum, polysaccharides, extraction, ultrasonic-assisted enzymatic hydrolysis, bioactivity

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