莱茵-埃农氏法和高效液相色谱-示差折光法检测奶粉中乳糖和蔗糖的比较研究

doi:10.7506/spkx1002-6630-201612024

食品科学

莱茵-埃农氏法和高效液相色谱-示差折光法检测奶粉中乳糖和蔗糖的比较研究

郭秀春，郭小白，张苗苗，马娇豪，康文艺,

1.河南大学药学院，中药研究所，河南开封 475004；2.开封市保健食品功效成分研究重点实验室，河南开封 475004；3.河南大学民生学院，河南开封 47500

出版日期:2016-06-25 发布日期:2016-06-29
通讯作者: 康文艺 E-mail:kangweny@hotmail.com
基金资助:
Lane-Eynon’s method; high performance liquid chromatography with refractive index detector (HPLC-RID);milk powder; lactose; sucrose

Comparative Detection of Lactose and Sucrose in Milk Powder by Lane-Eynon’s Method and High Performance Liquid Chromatography with Refractive Index Detector

GUO Xiuchun, GUO Xiaobai, ZHANG Miaomiao, MA Jiaohao, KANG Wenyi

1. Institute of Chinese Materia, Pharmaceutical College, Henan University, Kaifeng 475004, China;
2. Kaifeng Key Laboratory of Functional Components in Health Food, Kaifeng 475004, China;
3. Minsheng College, Henan University, Kaifeng 475004, China

Online:2016-06-25 Published:2016-06-29
Contact: KANG Wenyi E-mail:kangweny@hotmail.com

摘要/Abstract

摘要：

选用市售同种品牌不同年龄段的奶粉，采用莱茵-埃农氏法和高效液相色谱-示差折光检测法对其乳糖和蔗糖进行测定，分析乳糖和蔗糖的差异，并对两种分析方法的结果进行比较，为奶粉中乳糖和蔗糖的检测方法提供依据。结果表明，莱茵-埃农氏法和高效液相色谱-示差折光检测法相比，高效液相色谱-示差折光检测法测定乳糖和蔗糖含量的准确度更高，蔗糖和乳糖的线性范围均为2～12 mg/mL，相关系数分别为0.999 3、0.999 6，精密度实验结果表明，相对标准偏差分别为2.53%、3.81%，平均加样回收率分别为99.70%、101.06%。高效液相色谱-示差折光检测法结果准确、分析时间短、前处理简单，适用于快速测定奶粉中蔗糖和乳糖含量。

关键词: 莱茵-埃农氏法, 高效液相色谱-示差折光检测法, 奶粉, 蔗糖, 乳糖

Abstract:

Lactose and sucrose in milk powder from the same commercial brand for two different age groups were assayedby the Lane-Eynon’s method and high performance liquid chromatography with refractive index detector (HPLC-RID). The differences in lactose and sucrose contents among different samples were analyzed, and the results of the two methods were compared. It was indicated that the accuracy of the HPLC-RID method was higher than that of the Lane-Eynon’s method. The linear ranges of the HPLC method for sucrose and lactose were both 2–12 mg/mL with correlation coefficients of 0.999 3 and 0.999 6, respectively. The precision, expressed as relative standard deviations, for sucrose and lactose were 2.53% and3.81%, respectively. The average recovery rates were 99.70% and 101.06%, respectively. The HPLC method had some advantages such as high accuracy, short analysis time, simple sample pretreatment and suitability for rapid determination of sucrose and lactose contents in milk powder.

Key words: Lane-Eynon’s method, high performance liquid chromatography with refractive index detector (HPLC-RID);
milk powder, lactose, sucrose

中图分类号:

TS207.3

郭秀春，郭小白，张苗苗，马娇豪，康文艺,. 莱茵-埃农氏法和高效液相色谱-示差折光法检测奶粉中乳糖和蔗糖的比较研究[J]. 食品科学, doi: 10.7506/spkx1002-6630-201612024.

GUO Xiuchun, GUO Xiaobai, ZHANG Miaomiao, MA Jiaohao, KANG Wenyi. Comparative Detection of Lactose and Sucrose in Milk Powder by Lane-Eynon’s Method and High Performance Liquid Chromatography with Refractive Index Detector[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201612024.

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莱茵-埃农氏法和高效液相色谱-示差折光法检测奶粉中乳糖和蔗糖的比较研究

Comparative Detection of Lactose and Sucrose in Milk Powder by Lane-Eynon’s Method and High Performance Liquid Chromatography with Refractive Index Detector

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