食品科学

• 基础研究 • 上一篇    下一篇

UV-B辐照下大豆发芽富集异黄酮品种筛选及发芽动力学

陈 旭,杨润强,顾振新   

  1. 南京农业大学食品科技学院,江苏 南京 210095
  • 出版日期:2016-07-15 发布日期:2016-07-26
  • 通讯作者: 顾振新

Cultivar Selection for Isoflavone Accumulation and Kinetics of Soybean Germination under UV-B Radiation

CHEN Xu, YANG Runqiang, GU Zhenxin   

  1. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
  • Online:2016-07-15 Published:2016-07-26
  • Contact: GU Zhenxin

摘要:

以天骄(TJ)、苏青1号(SQ1)、苏青3号(SQ3)和95-优1(95Y1)4 个品种的大豆为原材料,在中波紫外光(ultraviolet radiation B,UV-B)辐照条件下,进行发芽实验,以发芽5 d后大豆异黄酮含量及其关键酶活力为主要指标,并综合分析生理生化指标,筛选出富集大豆异黄酮的最适合品种。结果表明:发芽的天骄大豆异黄酮含量、苯丙氨酸解氨酶(phenylalnine ammonialyase,PAL)和查耳酮合酶(chalcone synthase,CHS)活力最大,豆芽质量良好,发芽率、芽长、呼吸强度、游离氨基酸和还原糖含量都显著高于其余品种,故天骄大豆籽粒是发芽富集异黄酮的良好品种。以天骄为原料,对其进行发芽动力学实验。结果表明:随着发芽时间的延长,异黄酮含量显著增加,第7天为发芽前的3.02 倍,PAL活力显著上升,CHS活力先上升后下降。大豆中可溶性蛋白等大分子物质含量呈减少趋势,游离氨基酸等小分子物质含量逐渐增加。本实验筛选出天骄为UV-B辐照条件下富集大豆异黄酮的最适品种。

关键词: 大豆, 发芽, 异黄酮, UV-B辐照, 富集

Abstract:

Soybean cultivars Tian Jiao (TJ), Su Qing1 (SQ1), Su Qing3 (SQ3) and 95You1 (95Y1) were germinated
for 5 days under UV-B radiation to select the best cultivar for isoflavone accumulation based on the content of
isoflavone and the activity of key enzymes and comprehensive analysis of physio-biochemical indexes. Results
showed that sprouts of the TJ cultivar had the highest levels of isoflavone content, phenylalnine ammonialyase
(PAL) activity, and chalcone synthase (CHS) activity as well as the best quality. Meanwhile, the germination
percentage, sprout length, respiratory rate, and the contents of free amino acids and reduced sugar of the cultivar
TJ were higher than those of other tested cultivars. Thus, the cultivar TJ could be considered as a good source
for isoflavone accumulation. The content of isoflavone significantly increased with germination time, which was
3.02 times higher at 7 d than at 0 d. The activity of PAL likewise increased while the activity of CHS first increased and then
decreased. Macromolecular compounds such as soluble protein trended to decrease whereas small molecules such as free
amino acids increased gradually. In conclusion, TJ was screened out for isoflavone accumulation under UV-B radiation.

Key words: soybean, germination, isoflavone, UV-B radiation, accumulation

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