食品科学

• 成分分析 • 上一篇    下一篇

江香薷挥发油的化学成分分析及其对金黄色葡萄球菌生物被膜的抑制作用

李知敏,王 妹,彭 亮   

  1. 江西科技师范大学药学院,江西 南昌 330013
  • 出版日期:2016-07-25 发布日期:2016-07-26
  • 通讯作者: 彭 亮
  • 基金资助:

    江西省青年科学基金项目(20122BAB215017)

Chemical Composition Analysis of Essential Oil from Mosla chinensis Maxim. cv. Jiangxiangru and Inhibitory Activity of the Oil and Its Major Constituents on Biofilm Formation of Staphylococcus aureus

LI Zhimin, WANG Mei, PENG Liang   

  1. School of Pharmacy, Jiangxi Science and Technology Normal University, Nanchang 330013, China
  • Online:2016-07-25 Published:2016-07-26
  • Contact: PENG Liang

摘要:

目的:研究江香薷挥发油的化学成分及其对金黄色葡萄球菌生物被膜的影响。方法:运用气相色谱-质谱(gas chromatography-mass spectrometry,GC-MS)技术鉴定江香薷挥发油的化学成分,并对其中相对含量较大的8 种化学成分的金黄色葡萄球菌生物被膜抑制作用进行研究。结果:GC-MS分析鉴定出29 种成分,其中相对含量比较大的成分为百里香酚、香荆芥酚、对聚伞花素、γ-松油烯、百里香酚乙酸酯、α-石竹烯、3-蒈烯和香荆芥酚乙酸酯。江香薷挥发油对金黄色葡萄球菌生物被膜形成的抑制率可达91.3%,对成熟的金黄色葡萄球菌生物被膜清除率为78.5%,香荆芥酚、3-蒈烯、香荆芥酚乙酸酯和α-石竹烯对金黄色葡萄球菌生物被膜的形成抑制作用显著,其中3-蒈烯的抑制率最高,为93.9%,而对聚伞花素和百里香酚乙酸酯对成熟的金黄色葡萄球菌生物被膜清除作用比较明显。结论:江香薷挥发油对金黄色葡萄球菌生物被膜形成有较强的抑制作用,也具有一定的成熟金黄色葡萄球菌生物被膜清除作用,这可能是江香薷挥发油中多种化学成分共同作用的结果。

关键词: 江香薷, 挥发油, 气相色谱-质谱分析, 金黄色葡萄球菌生物被膜, 抑制作用

Abstract:

Objective: To analyze the chemical composition of the essential oil from Mosla chinensis Maxim. cv.
Jiangxiangru and to examine the inhibition of the oil and its main components on the biofilm formation of Staphylococcus
aureus. Methods: The chemical composition analysis was carried out by gas chromatography-mass spectrometry (GCMS).
The inhibitory effects of the essential oil and its eight main components were examined against the biofilm formation
of Staphylococcus aureus. Results: A total of 29 chemical compositions were identified in the essential oil. Its main
components were thymol, carvacrol, p-cymene, γ-terpenene, thymyl acetate, α-caryophyllene, 3-carene, and carvacryl
acetate. The percentage inhibition of the biofilm formation of Staphylococcus aureus by the essential oil reached 91.3%,
although scavenging only 78.5% of the mature biofilm of Staphylococcus aereus. Carvacrol, 3-carene, carvacryl acetate and
α-caryophyllene showed obvious inhibition against the formation of Staphylococcus aureus biofilms. Among these, 3-carene
was the most effective inhibitor with a percentage inhibition of 93.9%. On the other hand, both p-cymene and thymyl acetate
were able to significantly scavenge the mature biofilm of Staphylococcus aureus. Conclusion: The essential oil from Mosla
chinensis Maxim. cv. Jiangxiangru possessed a very strong inhibitory effect on the biofilm formation of Staphylococcus
aureus. Moreover, it also could scavenge the mature biofilm of Staphylococcus aureus. This may be a result of the combined
effects of several components in the essential oil.

Key words: Mosla chinensis Maxim. cv. Jiangxiangru, essential oil, gas chromatography-mass spectrometry (GC-MS) analysis, Staphylococcus aureus biofilms, inhibition

中图分类号: