食品科学

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蔗渣木聚糖含量检测方法的比较

苗 露,周玉恒,张厚瑞,郝再彬   

  1. 1.桂林理工大学化学与生物工程学院,广西 桂林 541006;
    2.广西植物研究所 广西植物功能物质研究与利用重点实验室,广西 桂林 541006
  • 出版日期:2016-08-25 发布日期:2016-08-30
  • 通讯作者: 张厚瑞
  • 基金资助:

    国家高技术研究发展计划(863计划)项目(2014AA021905B);
    广西植物功能物质研究与利用重点实验室主任基金项目(ZRJJ2013-1;ZRJJ2014-3;ZRJJ2015-1;ZRJJ2016-1);
    广西科学研究与技术开发计划项目(桂科重14122003-5;桂科重1348002-5);
    广西植物研究所基本业务项目(桂植业14011;桂植业16009);广西自然科学基金项目(2013jjBA30007)

Comparison of Analytical Methods for the Quantitation of Xylan in Sugarcane Bagasse

MIAO Lu, ZHOU Yuheng, ZHANG Hourui, HAO Zaibin   

  1. 1. College of Chemical and Biological Engineering, Guilin University of Technology, Guilin 541006, China;
    2. Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization, Guangxi Institute of Botany, Guilin 541006, China
  • Online:2016-08-25 Published:2016-08-30
  • Contact: ZHANG Hourui

摘要:

为建立一种准确评价木聚糖含量的方法,分别采用糖化法和醛化法,并辅助气相色谱-质谱色谱、高效液相色谱、比色和滴定等多种检测手段,对比检测同批次蔗渣木聚糖样品的含量。结果表明:醛化法只能检测木聚糖组分中的戊糖,漏检不能转化为糠醛的己糖,糖化法的3,5-二硝基水杨酸比色检测、高效液相钙离子柱检测和高效液相色谱柱前衍生检测所得的结果大体一致,即它们的测定结果都包括了木聚糖的戊糖与己糖组分,充分反映了木聚糖的真实糖含量,它们的结果之间具有可比性。单一使用钙离子色谱柱或单一使用柱前衍生高效液相色谱法检测,均存在个别单糖组分不能有效分离问题,但结合使用这2 种高效液相色谱法,可精确检测构成木聚糖的4 种单糖,即木糖、阿拉伯糖、半乳糖和葡萄糖。这种高效液相色谱检测组合适用于绝大多数植物木聚糖的糖类组成分析。

关键词: 蔗渣, 木聚糖, 含量, 检测

Abstract:

This study aimed to establish an accurate method for quantitatively determining xylan for the purpose
of broadening its range of application. A comparative evaluation of two sample pretreatment methods including
hydroformylation and hydrolytic saccharification respectively coupled with various analytical techniques such as gas
chromatography (GC), high performance liquid chromatography (HPLC), colorimetry and titrimetrywas was carried out for
the determination of xylan in the same batch of sugarcane bagasse. The results showed that the hydroformylation method
could only detect the pentose but it could not detect the hexose, which was unable to be converted into furfural. On the
other hand, by the combined use of hydrolytic saccharification with 3,5-dinitrosalicylic acid (DNS) colorimetry, HPLC with
Ca2+ column and pre-column derivatization HPLC, respectively, the results obtained were basically identical and authentic, that
is, both pentose and hexose could be detected using these methods. Single HPLC with Ca2+ column or pre-column derivatization
HPLC could not separate effectively some of the constituent monosaccharides. However, using a combination of the two HPLC
methods the four monosaccharides consisting of xylan could be accurately detected including xylose, arabinose, galactose and
glucose. This combination of HPLC methods is suitable for monosaccharide composition analysis of xylan in most plants.

Key words: sugar cane bagasse, xylan, contant, determination

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